Evidence map›Paper›PMID 29801343›Full record

ReviewOptics express2018

Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells [Invited].

Anna-Karin Gustavsson, Petar N Petrov, W E Moerner

Open access · goldAbstract readReview
In one paragraph

Review in Optics express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
8.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 66 citations in OpenAlex.

  1. Article
  2. Review
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  16. Review
  17. Multiresolution 3D Optical Mapping of Immune Cell Infiltrates in Mouse Asthmatic Lung.American journal of respiratory cell and molecular biology · 2023
    Article
  18. Article
  19. Article
  20. Single-molecule imaging in the primary cilium.Methods in cell biology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Anna-Karin Gustavsson
Petar N Petrov
W E Moerner
Stanford University · US

Funding

Single-Molecule Imaging for Cell Biology and Super-Resolution MicroscopyR35GM118067 · NIGMS · STANFORD UNIVERSITY · PI MOERNER, WILLIAM E · 2016 to 2025
$6.2M
Deep Super-localization Microscopy and Effectively Unbleachable Labeling for 4D NucleomicsU01EB021237 · NIBIB · STANFORD UNIVERSITY · PI LIPHARDT, JAN T. · 2015 to 2017
$1.1M
NIBIB NIH HHS U01 EB021237NIGMS NIH HHS R35 GM118067
6 · The paper itself

Abstract

The development of imaging techniques beyond the diffraction limit has paved the way for detailed studies of nanostructures and molecular mechanisms in biological systems. Imaging thicker samples, such as mammalian cells and tissue, in all three dimensions, is challenging due to increased background and volumes to image. Light sheet illumination is a method that allows for selective irradiation of the image plane, and its inherent optical sectioning capability allows for imaging of biological samples with reduced background, photobleaching, and photodamage. In this review, we discuss the advantage of combining single-molecule imaging with light sheet illumination. We begin by describing the principles of single-molecule localization microscopy and of light sheet illumination. Finally, we present examples of designs that successfully have married single-molecule super-resolution imaging with light sheet illumination for improved precision in mammalian cells.

Indexed as

Cellular StructuresAnimalsImaging, Three-DimensionalMicroscopy, Fluorescence

Identifiers

PMID29801343
PMCPMC6005674
OpenAlexW2799493475

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.