ArticleOptics express2018
Integrated one- and two-photon scanned oblique plane illumination (SOPi) microscopy for rapid volumetric imaging.
Article in Optics express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
59 citing papers in PubMed.
- Dual-scale and dual-channel oblique plane microscopy for multi-resolution imaging of large tissue specimens.Biomedical optics express · 2026Article
- Three-dimensional voltage imaging in live larval zebrafish brains using fully genetically encoded voltage indicator.Scientific reports · 2026Article
- Intravital mesoscale optical imaging: challenges, techniques, and future perspectives.Biophysics reports · 2026Article
- Mechanical de-skewing enables high-resolution imaging of thin tissue slices with a mesoSPIM light-sheet microscope.Biomedical optics express · 2026Article
- Rapid and gentle volumetric imaging of host-pathogen interactions in salmon skin cells using projective oblique plane microscopy.Scientific reports · 2025Article
- Increasing the acquisition speed in oblique plane microscopy via aliasing.Biomedical optics express · 2025Article
- Article
- Optical sectioning methods in three-dimensional bioimaging.Light, science & applications · 2025Review
- Mesoscopic axially swept oblique plane microscope for the imaging of freely moving organisms with near-isotropic resolution.Biomedical optics express · 2024Article
- Hyperspectral oblique plane microscopy enables spontaneous, label-free imaging of biological dynamic processes in live animals.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Frontiers in artificial intelligence-directed light-sheet microscopy for uncovering biological phenomena and multi-organ imaging.View (Beijing, China) · 2024Article
- Adaptive optics in an oblique plane microscope.Biomedical optics express · 2024Article
- User-friendly oblique plane microscopy on a fully functional commercially available microscope base.Biomedical optics express · 2024Article
- An end-to-end workflow for nondestructive 3D pathology.Nature protocols · 2024Review
- More than double the fun with two-photon excitation microscopy.Communications biology · 2024Review
- Adaptive Optics in an Oblique Plane Microscope.bioRxiv : the preprint server for biology · 2024Article
- S-polarized light-sheets improve resolution and light-efficiency in oblique plane microscopy.Scientific reports · 2024Article
- Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity.Nature communications · 2023Article
- Mesoscopic Oblique Plane Microscopy via Light-sheet Mirroring.bioRxiv : the preprint server for biology · 2023Article
- An end-to-end workflow for non-destructive 3D pathology.bioRxiv : the preprint server for biology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Versatile, sterically accessible imaging systems capable of in vivo rapid volumetric functional and structural imaging deep in the brain continue to be a limiting factor in neuroscience research. Towards overcoming this obstacle, we present integrated one- and two-photon scanned oblique plane illumination (SOPi, /sōpī/) microscopy which uses a single front-facing microscope objective to provide light-sheet scanning based rapid volumetric imaging capability at subcellular resolution. Our planar scan-mirror based optimized light-sheet architecture allows for non-distorted scanning of volume samples, simplifying accurate reconstruction of the imaged volume. Integration of both one-photon (1P) and two-photon (2P) light-sheet microscopy in the same system allows for easy selection between rapid volumetric imaging and higher resolution imaging in scattering media. Using SOPi, we demonstrate deep, large volume imaging capability inside scattering mouse brain sections and rapid imaging speeds up to 10 volumes per second in zebrafish larvae expressing genetically encoded fluorescent proteins GFP or GCaMP6s. SOPi's flexibility and steric access makes it adaptable for numerous imaging applications and broadly compatible with orthogonal techniques for actuating or interrogating neuronal structure and activity.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.