ArticleJournal of radiation research2018
Estimation of the radiation-induced DNA double-strand breaks number by considering cell cycle and absorbed dose per cell nucleus.
Article in Journal of radiation research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 39 citations in OpenAlex.
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- In vitro and in silico study of biological effects on cancer cells in the presence of metallic materials during radiotherapy.Journal of radiation research · 2024Article
- Cell-cycle dependence on the biological effects of boron neutron capture therapy and its modification by polyvinyl alcohol.Scientific reports · 2024Article
- The impact of dose rate on responses of human lens epithelial cells to ionizing irradiation.Scientific reports · 2024Article
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- Mechanistic modelling of oxygen enhancement ratio of radiation via Monte Carlo simulation-based DNA damage calculation.Physics in medicine and biology · 2022Article
- The potential use of tideglusib as an adjuvant radio-therapeutic treatment for glioblastoma multiforme cancer stem-like cells.Pharmacological reports : PR · 2021Article
- Variation of 4 MV X-ray dose rate strongly impacts biological response both in vitro and in vivo.Scientific reports · 2020Article
- A Simplified Cluster Analysis of Electron Track Structure for Estimating Complex DNA Damage Yields.International journal of molecular sciences · 2020Article
- A study on legal and medical dimensions of radiation exposure in neurosurgery clinics in Turkish practice.Surgical neurology international · 2020Article
- Track Structure Study for Energy Dependency of Electrons and X-rays on DNA Double-Strand Break Induction.Scientific reports · 2019Article
- Multiparametric radiobiological assays show that variation of X-ray energy strongly impacts relative biological effectiveness: comparison between 220 kV and 4 MV.Scientific reports · 2019Article
- DNA methylation changes following DNA damage in prostate cancer cells.Epigenetics · 2019Article
- DNA damage induction during localized chronic exposure to an insoluble radioactive microparticle.Scientific reports · 2019Article
- Analysis of the high-dose-range radioresistance of prostate cancer cells, including cancer stem cells, based on a stochastic model.Journal of radiation research · 2019Article
- Effect of Low-Dose Gamma Radiation and Lipoic Acid on High- Radiation-Dose Induced Rat Brain Injuries.Dose-response : a publication of International Hormesis SocietyArticle
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA double-strand breaks (DSBs) are thought to be the main cause of cell death after irradiation. In this study, we estimated the probability distribution of the number of DSBs per cell nucleus by considering the DNA amount in a cell nucleus (which depends on the cell cycle) and the statistical variation in the energy imparted to the cell nucleus by X-ray irradiation. The probability estimation of DSB induction was made following these procedures: (i) making use of the Chinese Hamster Ovary (CHO)-K1 cell line as the target example, the amounts of DNA per nucleus in the logarithmic and the plateau phases of the growth curve were measured by flow cytometry with propidium iodide (PI) dyeing; (ii) the probability distribution of the DSB number per cell nucleus for each phase after irradiation with 1.0 Gy of 200 kVp X-rays was measured by means of γ-H2AX immunofluorescent staining; (iii) the distribution of the cell-specific energy deposition via secondary electrons produced by the incident X-rays was calculated by WLTrack (in-house Monte Carlo code); (iv) according to a mathematical model for estimating the DSB number per nucleus, we deduced the induction probability density of DSBs based on the measured DNA amount (depending on the cell cycle) and the calculated dose per nucleus. The model exhibited DSB induction probabilities in good agreement with the experimental results for the two phases, suggesting that the DNA amount (depending on the cell cycle) and the statistical variation in the local energy deposition are essential for estimating the DSB induction probability after X-ray exposure.
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