Evidence map›Paper›PMID 29798531›Full record

ArticleZhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery2017

[Effects of cryopreservation on biological characteristics of tendon-derived stem cells in rat patellar tendon].

Guangchun Dai, Yingjuan Li, Hongliang Xu, Yucheng Lin, Junyan Liu, Lin Xu, Yunfeng Rui

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 67% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. [Electrospun polycaprolactone/collagen typeZhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Guangchun DaiDepartment of Orthpaedics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing Jiangsu, 210000, P.R.China;School of Medicine, Southeast University, Nanjing Jiangsu, 210000, P.R.China.
Yingjuan LiDepartment of Geriatrics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing Jiangsu, 210000, P.R.China.
Hongliang XuDepartment of Orthopaedics, Wuxi Branch, Zhongda Hospital, School of Medicine, Southeast University, Wuxi Jiangsu, 214000, P.R.China.
Yucheng LinDepartment of Orthpaedics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing Jiangsu, 210000, P.R.China.
Junyan LiuDepartment of Orthpaedics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing Jiangsu, 210000, P.R.China;School of Medicine, Southeast University, Nanjing Jiangsu, 210000, P.R.China.
Lin XuDepartment of Orthopaedics, Wuxi Branch, Zhongda Hospital, School of Medicine, Southeast University, Wuxi Jiangsu, 214000, P.R.China.
Yunfeng RuiDepartment of Orthpaedics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing Jiangsu, 210000, P.R.China;School of Medicine, Southeast University, Nanjing Jiangsu, 210000, P.R.China;Department of Orthopaedics, Wuxi Branch, Zhongda Hospital, School of Medicine, Southeast University, Wuxi Jiangsu, 214000, P.R.China.ruiyunfeng@126.com.
Southeast University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: To explore the effects of cryopreservation on the cell survival rate, cell viability, early apoptosis, migration ability, and tendon-related marker expression of tendon-derived stem cells (TDSCs) in rat patellar tendons. Methods: The patellar tendon tissues were harvested from 12 4-month-old male Sprague Dawley rats; 12 patellar tendon tissues from 6 rats were cryopreserved (the experimental group), and the other 12 patellar tendon tissues were not treated (the control group). The patellar tendons were digested with 0.3% type I collagenase to obtain nucleated cells. The survival rate of nucleated cells was detected by trypan blue exclusion assay, and colony-forming ability by crystal violet staining. TDSCs were isolated and cultured to passage 3 (P3). The cell viability of TDSCs was detected by Alamar Blue method, the early apoptosis by Annexin V-FITC/PI assay, the cell migration ability by Transwell method, and the mRNA expressions of tendon-related markers [collagen type I (Col1α1), scleraxis (Scx), and tenomodulin (Tnmd)] by real-time quantitative PCR. Results: The survival rate of nucleated cells was 91.00%±3.63% in the control group, and was 61.65%±4.76% in the experimental group, showing significant difference ( Conclusion: The survival rate of nucleated cells in cryopreserved rat tendon tissues is lower, but a large number of active TDSCs, and its cell viability, early apoptosis rate, migration ability

Indexed as

CryopreservationStem CellsTendonsAnimalsCell DifferentiationCells, CulturedMaleMembrane ProteinsPatellar LigamentRatsRats, Sprague-DawleyMembrane ProteinsTnmd protein, ratallogeneic tendon transplantationCryopreservationtendon defecttendon-derived stem cellstenogenic differentiation

Identifiers

PMID29798531
PMCPMC8498150
OpenAlexW2985694236

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.