ArticleTransgenic research2018
CRISPR/Cas9 editing of carotenoid genes in tomato.
Article in Transgenic research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 97 citations in OpenAlex.
- Exploring and exploiting genetics and genomics for sweetpotato improvement: Status and perspectives.Plant communications · 2022Pooled it
- Genome Editing in Solanaceae: Harnessing CRISPR-Cas Technology for Precision Crop Improvement.Plants (Basel, Switzerland) · 2026Review
- Cultivar-Dependent Biosynthesis, Sustainable Recovery, and Industrial Applications of Tomato-Derived Carotenoids.Plants (Basel, Switzerland) · 2026Review
- A duplex sequencing approach for high-sensitivity detection of genome-edited plants.Food chemistry. Molecular sciences · 2025Article
- CRISPR mediated gene editing for economically important traits in horticultural crops: progress and prospects.Transgenic research · 2025Review
- Recent Advances in Tomato Gene Editing.International journal of molecular sciences · 2024Review
- Review
- Improvement of crop production in controlled environment agriculture through breeding.Frontiers in plant science · 2024Review
- Overlapping and specialized roles of tomato phytoene synthases in carotenoid and abscisic acid production.Plant physiology · 2023Article
- CRISPR/Cas9 gene editing technology: a precise and efficient tool for crop quality improvement.Planta · 2023Review
- Genome editing technologies, mechanisms and improved production of therapeutic phytochemicals: Opportunities and prospects.Biotechnology and bioengineering · 2023Review
- Characterization of prolycopene-accumulated TanJournal of biosciences · 2023Article
- Metabolic engineering of plant secondary metabolites: prospects and its technological challenges.Frontiers in plant science · 2023Review
- CRISPR/Cas genome editing in tomato improvement: Advances and applications.Frontiers in plant science · 2023Review
- CRISPR/Cas 9-Based Editing in the Production of Bioactive Molecules.Molecular biotechnology · 2022Review
- CRISPR/Cas9 and Nanotechnology Pertinence in Agricultural Crop Refinement.Frontiers in plant science · 2022Review
- Comprehending the evolution of gene editing platforms for crop trait improvement.Frontiers in genetics · 2022Review
- Genome editing in fruit, ornamental, and industrial crops.Transgenic research · 2021Review
- Inhibition of Carotenoid Biosynthesis by CRISPR/Cas9 Triggers Cell Wall Remodelling in Carrot.International journal of molecular sciences · 2021Article
- Application of CRISPR/Cas9 in Crop Quality Improvement.International journal of molecular sciences · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas9 technology is rapidly spreading as genome editing system in crop breeding. The efficacy of CRISPR/Cas9 in tomato was tested on Psy1 and CrtR-b2, two key genes of carotenoid biosynthesis. Carotenoids are plant secondary metabolites that must be present in the diet of higher animals because they exert irreplaceable functions in important physiological processes. Psy1 and CrtR-b2 were chosen because their impairment is easily detectable as a change of fruit or flower color. Two CRISPR/Cas9 constructs were designed to target neighboring sequences on the first exon of each gene. Thirty-four out of forty-nine (69%) transformed plants showed the expected loss-of-function phenotypes due to the editing of both alleles of a locus. However, by including the seven plants edited only at one of the two homologs and showing a normal phenotype, the editing rate reaches the 84%. Although none chimeric phenotype was observed, the cloning of target region amplified fragments revealed that in the 40% of analyzed DNA samples were present more than two alleles. As concerning the type of mutation, it was possible to identify 34 new different alleles across the four transformation experiments. The sequence characterization of the CRISPR/Cas9-induced mutations showed that the most frequent repair errors were the insertion and the deletion of one base. The results of this study prove that the CRISPRCas9 system can be an efficient and quick method for the generation of useful mutations in tomato to be implemented in breeding programs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.