Evidence map›Paper›PMID 29797090›Full record

ArticleExtremophiles : life under extreme conditions2018

Native xylose-inducible promoter expands the genetic tools for the biomass-degrading, extremely thermophilic bacterium Caldicellulosiruptor bescii.

Amanda M Williams-Rhaesa, Nanaakua K Awuku, Gina L Lipscomb, Farris L Poole, Gabriel M Rubinstein, Jonathan M Conway, Robert M Kelly, Michael W W Adams

Abstract read
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In one paragraph

Article in Extremophiles : life under extreme conditions, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
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  5. Current models in bacterial hemicellulase-encoding gene regulation.Applied microbiology and biotechnology · 2024
    Review
  6. ACS synthetic biology · 2024
    Article
  7. The potential of native and engineered Clostridia for biomass biorefining.Frontiers in bioengineering and biotechnology · 2024
    Review
  8. Article
  9. Article
  10. Review
  11. Review
  12. Article
  13. Differential Thermotolerance Adaptation between Species ofJournal of fungi (Basel, Switzerland) · 2020
    Article
  14. Review
  15. Review
  16. The thermophilic biomass-degrading bacteriumThe Journal of biological chemistry · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Amanda M Williams-RhaesaDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA.
Nanaakua K AwukuDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA.
Gina L LipscombDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA.
Farris L PooleDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA.
Gabriel M RubinsteinDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA.
Jonathan M ConwayDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, USA.
Robert M KellyDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, USA.
Michael W W AdamsDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA. adamsm@uga.edu.
University of Georgia · USNorth Carolina State University · US

Funding

US Department of Energy DE-PS02-06ER64304
6 · The paper itself

Abstract

Regulated control of both homologous and heterologous gene expression is essential for precise genetic manipulation and metabolic engineering of target microorganisms. However, there are often no options available for inducible promoters when working with non-model microorganisms. These include extremely thermophilic, cellulolytic bacteria that are of interest for renewable lignocellulosic conversion to biofuels and chemicals. In fact, improvements to the genetic systems in these organisms often cease once transformation is achieved. This present study expands the tools available for genetically engineering Caldicellulosiruptor bescii, the most thermophilic cellulose-degrader known growing up to 90 °C on unpretreated plant biomass. A native xylose-inducible (P

Indexed as

Promoter Regions, GeneticBacillotaBiotransformationCelluloseIndustrial MicrobiologyL-Lactate DehydrogenaseThermotoleranceXyloseCelluloseL-Lactate DehydrogenaseXyloseAnaerobesBiodegradation of cellulosicBiotechnology of thermophilesGeneticsGenetics of extremophilesMolecular biology

Identifiers

PMID29797090
OpenAlexW2803730237

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.