Evidence map›Paper›PMID 29796058›Full record

ArticleCell division2018

Eg5 orchestrates porcine oocyte maturational progression by maintaining meiotic organelle arrangement.

Yan Xie, Minghui Cheng, Shan Lu, Qilong Yuan, Dongyu Yang, Ying Chen, Chen Pan, Yurong Qiu, Bo Xiong

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell division, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Yan Xie1Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, 510515 China.
Minghui Cheng3College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 China.
Shan Lu2Department of Reproductive Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120 China.
Qilong Yuan2Department of Reproductive Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120 China.
Dongyu Yang2Department of Reproductive Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120 China.
Ying Chen3College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 China.
Chen Pan3College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 China.
Yurong Qiu1Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, 510515 China.
Bo Xiong3College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 China.
Nanjing Agricultural University · CNGuangzhou University of Chinese Medicine · CNNanfang Hospital · CNSouthern Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundKinesin superfamily proteins are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. However, their exact roles during mammalian oocyte meiosis have not been fully clarified.

resultsHerein, we investigated the critical events during porcine oocyte meiotic maturation with the treatment of Eg5-specific inhibitor monastrol. We found that Eg5 inhibition resulted in oocyte meiotic failure by displaying the poor expansion of cumulus cells and reduced rate of polar body extrusion. In the meantime, the spindle assembly and chromosome alignment were compromised, accompanied by the decreased level of acetylated α-tubulin, indicative of less stable microtubules. Impaired actin dynamics and mitochondria integrity were also observed in Eg5-inhibited oocytes. Additionally, inhibition of Eg5 caused the abnormal distribution of cortical granules and ovastacin, a cortical granule component, potentially leading to the fertilization failure.

conclusionsOur findings reveal that Eg5 possesses an important function in porcine oocyte meiotic progression by regulating the organelle dynamics and arrangement.

Indexed as

Actin dynamicsCortical granuleMitochondrion integrityOocyte meiotic maturationSpindle assembly

Identifiers

PMID29796058
PMCPMC5966870
OpenAlexW2806299105

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.