ArticlemSystems
Viromic Analysis of Wastewater Input to a River Catchment Reveals a Diverse Assemblage of RNA Viruses.
Article in mSystems. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 87 citations in OpenAlex.
- Optimizing methods for virome analysis based on studies of a synthetic viral community.mSystems · 2026Article
- Picobirnavirus: how do you find where it's hiding?Critical reviews in microbiology · 2026Review
- Optimizing methods for virome analysis based on studies of a synthetic viral community.bioRxiv : the preprint server for biology · 2025Article
- Recovering wastewater RNA for virome sequencing by systematically optimized tangential-flow ultrafiltration and Nanotrap microbiome particles.Applied and environmental microbiology · 2025Article
- Wastewater Metavirome Diversity: Exploring Replicate Inconsistencies and Bioinformatic Tool Disparities.International journal of environmental research and public health · 2025Article
- Advancing RNA phage biology through meta-omics.Nucleic acids research · 2025Review
- Assessing Bias and Reproducibility of Viral Metagenomics Methods for the Combined Detection of Faecal RNA and DNA Viruses.Viruses · 2025Article
- Comparison of Three Viral Nucleic Acid Preamplification Pipelines for Sewage Viral Metagenomics.Food and environmental virology · 2024Article
- Article
- Genomic and phylogenetic features of theVirus evolution · 2024Article
- RNA-Seq Virus Fraction in Lake Baikal and Treated Wastewaters.International journal of molecular sciences · 2023Article
- Analysis of metatranscriptomic methods to enable wastewater-based biosurveillance of all infectious diseases.Frontiers in public health · 2023Article
- Putative Invertebrate, Plant, and Wastewater Derived ssRNA Viruses in Plankton of the Anthropogenically Impacted Anacostia River, District of Columbia, USA.Microbes and environments · 2022Article
- Reduction and discharge of SARS-CoV-2 RNA in Chicago-area water reclamation plants.FEMS microbes · 2022Article
- RNA-viromics reveals diverse communities of soil RNA viruses with the potential to affect grassland ecosystems across multiple trophic levels.ISME communications · 2022Article
- Temporal variations of human and animalFrontiers in microbiology · 2022Article
- Feasibility of neighborhood and building scale wastewater-based genomic epidemiology for pathogen surveillance.The Science of the total environment · 2021Article
- Occurrence of various viruses and recent evidence of SARS-CoV-2 in wastewater systems.Journal of hazardous materials · 2021Review
- Metagenomic and viromic data mining reveals viral threats in biologically treated domestic wastewater.Environmental science and ecotechnology · 2021Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detection of viruses in the environment is heavily dependent on PCR-based approaches that require reference sequences for primer design. While this strategy can accurately detect known viruses, it will not find novel genotypes or emerging and invasive viral species. In this study, we investigated the use of viromics, i.e., high-throughput sequencing of the biosphere's viral fraction, to detect human-/animal-pathogenic RNA viruses in the Conwy river catchment area in Wales, United Kingdom. Using a combination of filtering and nuclease treatment, we extracted the viral fraction from wastewater and estuarine river water and sediment, followed by high-throughput RNA sequencing (RNA-Seq) analysis on the Illumina HiSeq platform, for the discovery of RNA virus genomes. We found a higher richness of RNA viruses in wastewater samples than in river water and sediment, and we assembled a complete norovirus genotype GI.2 genome from wastewater effluent, which was not contemporaneously detected by conventional reverse transcription-quantitative PCR (qRT-PCR). The simultaneous presence of diverse rotavirus signatures in wastewater indicated the potential for zoonotic infections in the area and suggested runoff from pig farms as a possible origin of these viruses. Our results show that viromics can be an important tool in the discovery of pathogenic viruses in the environment and can be used to inform and optimize reference-based detection methods provided appropriate and rigorous controls are included.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.