ArticleScientific reports2018
Concentration-dependent protein loading of extracellular vesicles released by Histoplasma capsulatum after antibody treatment and its modulatory action upon macrophages.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 81 citations in OpenAlex.
- Comparative Proteomics of Extracellular Vesicles of Two Species of Mucorales Reveals Species-Specific Cargo.Mycopathologia · 2026Article
- Extracellular vesicles in human fungal pathogens: Biogenesis, functions, and translational applications.Journal of microbiology (Seoul, Korea) · 2026Review
- Lectin-Fc(IgG2a) fusion proteins binding to the cell wall ofMicrobiology spectrum · 2026Article
- Extracellular vesicles ofInfection and immunity · 2026Article
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- How Histoplasma Evades the Human Immune System.Acta medica academica · 2025Review
- Extracellular vesicles from DistinctACS infectious diseases · 2025Article
- Efficacy of nitrofuran derivatives against biofilms ofFuture microbiology · 2025Article
- The impact of Aureobasidium melanogenum cells and extracellular vesicles on human cell lines.Scientific reports · 2025Article
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- Influence of Zinc onJournal of fungi (Basel, Switzerland) · 2024Article
- Human-Fungal Pathogen Interactions from the Perspective of Immunoproteomics Analyses.International journal of molecular sciences · 2024Review
- Extracellular Vesicles and Immunity: At the Crossroads of Cell Communication.International journal of molecular sciences · 2024Review
- Yeast strains isolated from fermented beverage produce extracellular vesicles with anti-inflammatory effects.Scientific reports · 2024Article
- Traversing the Cell Wall: The Chitinolytic Activity ofJournal of fungi (Basel, Switzerland) · 2023Article
- Immunomodulatory Potential of Fungal Extracellular Vesicles: Insights for Therapeutic Applications.Biomolecules · 2023Review
- Characteristics and potential clinical applications of the extracellular vesicles of human pathogenic Fungi.BMC microbiology · 2023Review
- Extracellular Vesicles fromJournal of fungi (Basel, Switzerland) · 2023Article
- Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
Abstract
Diverse pathogenic fungi secrete extracellular vesicles (EV) that contain macromolecules, including virulence factors that can modulate the host immune response. We recently demonstrated that the binding of monoclonal antibodies (mAb) modulates how Histoplasma capsulatum load and releases its extracellular vesicles (EV). In the present paper, we addressed a concentration-dependent impact on the fungus' EV loading and release with different mAb, as well as the pathophysiological role of these EV during the host-pathogen interaction. We found that the mAbs differentially regulate EV content in concentration-dependent and independent manners. Enzymatic assays demonstrated that laccase activity in EV from H. capsulatum opsonized with 6B7 was reduced, but urease activity was not altered. The uptake of H. capsulatum by macrophages pre-treated with EV, presented an antibody concentration-dependent phenotype. The intracellular killing of yeast cells was potently inhibited in macrophages pre-treated with EV from 7B6 (non-protective) mAb-opsonized H. capsulatum and this inhibition was associated with a decrease in the reactive-oxygen species generated by these macrophages. In summary, our findings show that opsonization quantitatively and qualitatively modifies H. capsulatum EV load and secretion leading to distinct effects on the host's immune effector mechanisms, supporting the hypothesis that EV sorting and secretion are dynamic mechanisms for a fine-tuned response by fungal cells.
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Registered trials
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