ArticleScientific reports2018
Extraction and quantification of biofilm bacteria: Method optimized for urinary catheters.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
57 citing papers in PubMed, 121 citations in OpenAlex.
- RK-14: A Novel Antimicrobial Peptide Targeting Multidrug-Resistant Bacteria and Catheter-Associated Biofilms.Probiotics and antimicrobial proteins · 2026Article
- Repression of the Type VI Secretion System (T6SS) During Biofilm Formation Provides a Fitness Advantage forMicroorganisms · 2026Article
- A Standardised Combinational Method for Evaluating Antimicrobial Compounds Against Biofilm Attachment, Development and Eradication.Microorganisms · 2026Article
- Identifying markers of biofilm formation on medical-grade stainless steel as a representative medical device material.Microbiology (Reading, England) · 2026Article
- Building evidence for transfusion practice: A pilot study on bacterial adhesion and growth in red blood cell infusion sets.Transfusion · 2026Article
- Biofilm formation on venovenous ECMO cannulas can lead to re-introduction of pathogens during the decannulation process - a small-scale study reveals new insights when combining cultures and molecular results.BMC infectious diseases · 2026Observational
- Surface Properties of Dental Materials Influence the In Vitro Multi-Species Biofilm Formation.Polymers · 2026Article
- Tackling Drug Resistance and Virulence inThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2026Article
- Sanitization of food-grade materials: Combined effect of an enzymatic product and two chemical sanitizers onAIMS microbiology · 2026Article
- A Novel Approach for Tissue Analysis in Joint Infections Using the Scattered Light Integrating Collector (SLIC).Biosensors · 2025Article
- Polymicrobial Biofilms: Interkingdom Interactions, Resistance and Therapeutic Strategies.Microbial biotechnology · 2025Review
- Control ofFuture microbiology · 2025Article
- Efficacy of phage vB_Ps_ZCPS13 in controlling Pan-drug-resistant Pseudomonas aeruginosa from urinary tract infections (UTIs) and eradicating biofilms from urinary catheters.Virology journal · 2025Article
- Fighting biofilm: bacteriophages eliminate biofilm formed by multidrug-resistant Enterobacter hormaechei on urological catheters.Medical microbiology and immunology · 2025Article
- Advantages in orthopaedic implant infection diagnostics by additional analysis of explants.International orthopaedics · 2025Article
- Proteomic insights into dual-species biofilm formation of E. coli and E. faecalis on urinary catheters.Scientific reports · 2025Article
- Virulence traits and bacterial interactions within the complex microbial population in urinary double-J catheters.Frontiers in microbiology · 2025Article
- Article
- Multi-Functional Biomaterial for the Treatment and Prevention of Central Line-Associated Bloodstream Infections.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial biofilms are responsible for the failure of many medical devices such as urinary catheters and are associated with many infectious and non-infectious complications. Preclinical and clinical evaluation of novel catheter coatings to prevent these infections needs to accurately quantify the bacterial load in the biofilm in vitro and ex vivo. There is currently no uniform gold standard for biofilm quantification for different surfaces and established biofilms. We have tried to establish a simple, accurate and reproducible method for extraction and measurement of biofilm bacteria on indwelling catheters, using a combination of vortexing and sonication. We demonstrate the usefulness of this method for catheters of different sizes - 3 Fr to 14 Fr - in vitro, in murine and porcine models, and indwelling in human clinical subjects. We also demonstrate consistent results with complex and polymicrobial biofilms. We believe that this standardized reproducible method will assist the assessment of biofilms in general and urological devices in particular in efforts to harness novel technologies to prevent healthcare associated infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.