Evidence map›Paper›PMID 29794135›Full record

ArticleThe Journal of biological chemistry2018

General method for rapid purification of native chromatin fragments.

Vyacheslav I Kuznetsov, Spencer A Haws, Catherine A Fox, John M Denu

Abstract readEvaluation Study
In one paragraph

Article in The Journal of biological chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. Potent Activation of NADACS chemical biology · 2022
    Article
  9. Article
  10. Review
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Vyacheslav I KuznetsovDepartment of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53715; Wisconsin Institute for Discovery, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53715.
Spencer A HawsDepartment of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53715; Wisconsin Institute for Discovery, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53715.
Catherine A FoxDepartment of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53715. Electronic address: cfox@wisc.edu.
John M DenuDepartment of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53715; Wisconsin Institute for Discovery, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53715; Morgridge Institute for Research, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53715. Electronic address: john.denu@wisc.edu.

Funding

TRAINING IN NUTRITIONT32DK007665 · NIDDK · UNIVERSITY OF WISCONSIN-MADISON · PI GUY E GROBLEWSKI, Chi- Liang Eric Yen · 1993 to 2026
$9.2M
Origin Recognition Complex at Transcriptional SilencersR01GM056890 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI FOX, CATHERINE A · 1998 to 2020
$6.4M
Molecular mechanisms of histone modification - Alzheimer’s supplement NOT-AG-18-008R37GM059785 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI DENU, JOHN M · 2013 to 2022
$3.7M
Molecular Mechanisms of Histone AcetyltransferasesR01GM059785 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI DENU, JOHN M · 1999 to 2012
$2.9M
NIDDK NIH HHS T32 DK007665NIGMS NIH HHS R01 GM056890NIGMS NIH HHS R01 GM059785NIGMS NIH HHS R37 GM059785
6 · The paper itself

Abstract

Biochemical, proteomic, and epigenetic studies of chromatin rely on the ability to efficiently isolate native nucleosomes in high yield and purity. However, isolation of native chromatin suitable for many downstream experiments remains a challenging task. This is especially true for the budding yeast

Indexed as

Cell NucleusChemistry Techniques, AnalyticalChromatinChromatography, Ion ExchangeSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsChromatinSaccharomyces cerevisiae Proteinsacetylationchromatinchromatin isolationchromatin modificationchromatin structurechromatographyepigeneticshistoneinsect cellmass spectrometryMCF-7methylationnative chromatin fragmentsnucleosomeoligonucleosomespost-translational modification (PTM)purificationyeast

Identifiers

PMID29794135
PMCPMC6078465

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.