Evidence map›Paper›PMID 29793367›Full record

ArticleInhalation toxicology2018

Dimethylarginine dimethylaminohydrolase (DDAH) overexpression enhances wound repair in airway epithelial cells exposed to agricultural organic dust.

Deepak Chandra, Jill A Poole, Kristina L Bailey, Elizabeth Staab, Jenea M Sweeter, Jane M DeVasure, Debra J Romberger, Todd A Wyatt

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Inhalation toxicology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. The effect ofVeterinary world · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Deepak Chandraa Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine , University of Nebraska Medical Center , Omaha , NE Douglas.
Jill A Poolea Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine , University of Nebraska Medical Center , Omaha , NE Douglas.
Kristina L Baileya Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine , University of Nebraska Medical Center , Omaha , NE Douglas.
Elizabeth Staaba Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine , University of Nebraska Medical Center , Omaha , NE Douglas.
Jenea M Sweetera Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine , University of Nebraska Medical Center , Omaha , NE Douglas.
Jane M DeVasurea Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine , University of Nebraska Medical Center , Omaha , NE Douglas.
Debra J Rombergera Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine , University of Nebraska Medical Center , Omaha , NE Douglas.
Todd A Wyatta Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine , University of Nebraska Medical Center , Omaha , NE Douglas.ORCID 0000-0002-9273-6730
University of Nebraska Medical Center · USVA Nebraska Western Iowa Health Care System · USNebraska Medical Center · US

Funding

Surveillance of Agricultural Injuries in the Central States RegionsU54OH010162 · OH · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI RAUTIAINEN, RISTO HEIKKI · 2011 to 2025
$23.6M
Role of pattern recognition receptors in organic dust-induced airway inflammationR01ES019325 · NIEHS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI POOLE, JILL A · 2010 to 2020
$3.7M
Targeting Airway Inflammation from Concentrated Animal Feeding Operation DustR01OH008539 · OH · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI ROMBERGER, DEBRA J · 2006 to 2014
$3.7M
Airway Injury Caused by MAA AdductsR01AA017993 · NIAAA · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI WYATT, TODD A · 2008 to 2012
$1.9M
Mucociliary clearance in agingR01AG053553 · NIA · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI BAILEY, KRISTINA L · 2016 to 2020
$1.5M
Malondialdehyde-acetaldehyde adducts and lung injuryI01BX003635 · VA · OMAHA VA MEDICAL CENTER · PI TED RICHARD MIKULS · 2017 to 2026
–
BLR&D Research Career Scientist AwardIK6BX003781 · VA · OMAHA VA MEDICAL CENTER · PI WYATT, TODD A · 2017 to 2021
–
ACL HHS U54OH010162BLRD VA I01 BX003635BLRD VA IK6 BX003781NIAAA NIH HHS R01 AA017993NIA NIH HHS R01 AG053553NIEHS NIH HHS R01 ES019325NIOSH CDC HHS R01 OH008539NIOSH CDC HHS U54 OH010162
6 · The paper itself

Abstract

objectiveWorkers exposed to dusts from concentrated animal feeding operations have a high prevalence of pulmonary diseases. These exposures lead to chronic inflammation and aberrant airway remodeling. Previous work shows that activating cAMP-dependent protein kinase (PKA) enhances airway epithelial wound repair while activating protein kinase C (PKC) inhibits wound repair. Hog barn dust extracts slow cell migration and wound repair via a PKC-dependent mechanism. Further, blocking nitric oxide (NO) production in bronchial epithelial cells prevents PKA activation. We hypothesized that blocking an endogenous NO inhibitor, asymmetric dimethylarginine, by overexpressing dimethylarginine dimethylaminohydrolase mitigates the effects of hog dust extract on airway epithelial would repair. MATERIALS/

methodsWe cultured primary tracheal epithelial cells in monolayers from both wild-type (WT) and dimethylarginine dimethylaminohydrolase overexpressing C57Bl/6 (DDAH

resultsWound closure in epithelial cells from WT mice occurred within 24 h in vitro. In contrast, treatment of the WT cell monolayers with 5% hog dust extract prevented significant NO-stimulated wound closure. In cells from DDAH DISCUSSION/

conclusionPreserving the NO signal through endogenous inhibition of asymmetric dimethylarginine enhances wound repair even in the presence of dust exposure.

Indexed as

Animal HusbandryDustWound HealingAmidohydrolasesAnimalsArginineCells, CulturedCyclic AMP-Dependent Protein KinasesEpithelial CellsMice, Inbred C57BLMice, TransgenicNitric OxideProtein Kinase CTracheaAmidohydrolasesArginineCyclic AMP-Dependent Protein KinasesdimethylargininaseDustNitric OxideN,N-dimethylarginineProtein Kinase Cdimethylarginine dimethylaminohydrolaseEpithelial wound repairnitric oxideprotein kinase C

Identifiers

PMID29793367
PMCPMC6176920
OpenAlexW2804405295

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.