ArticleJournal of neuroscience methods2018
t-GRASP, a targeted GRASP for assessing neuronal connectivity.
Article in Journal of neuroscience methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
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- A new bridge from sleep to wake in fruit flies.Sleep · 2026Article
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- SIFamide-dependent synaptic plasticity in male-specific GABAergic neurons underlies experience-modulated mating behaviors.iScience · 2025Article
- A brief history of insect neuropeptide and peptide hormone research.Cell and tissue research · 2025Review
- Monitoring Synapses via Trans-synaptic GFP Complementation.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Visualization of Synapses in Larval Stages of Drosophila melanogaster Using the GRASP Technique.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Constitutive and Conditional Epitope Tagging of Endogenous G-Protein-Coupled Receptors inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Tango-seq: overlaying transcriptomics on connectomics to identify neurons downstream ofbioRxiv : the preprint server for biology · 2024Article
- Mechanosensory and command contributions to the Drosophila grooming sequence.Current biology : CB · 2024Article
- Functional labeling of individualized postsynaptic neurons using optogenetics and trans-Tango in Drosophila (FLIPSOT).PLoS genetics · 2024Article
- Heterogeneity in the projections and excitability of tyraminergic/octopaminergic neurons that innervate theFrontiers in molecular neuroscience · 2024Article
- Biased cell adhesion organizes a circuit for visual motion integration.bioRxiv : the preprint server for biology · 2023Article
- Neuronal substrates of egg-laying behaviour at the abdominal ganglion of Drosophila melanogaster.Scientific reports · 2023Article
- Mechanosensory and command contributions to thebioRxiv : the preprint server for biology · 2023Article
- The synaptic basis for sexual dimorphism in the invertebrate nervous system.Current opinion in neurobiology · 2023Review
- Dietary cysteine drives body fat loss via FMRFamide signaling in Drosophila and mouse.Cell research · 2023Article
- Article
- Direction Selectivity of TmY Neurites in Drosophila.Neuroscience bulletin · 2023Article
- Article
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5 authors.
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Abstract
backgroundUnderstanding how behaviors are generated by neural circuits requires knowledge of the synaptic connections between the composite neurons. Methods for mapping synaptic connections, such as electron microscopy and paired recordings, are labor intensive and alternative methods are thus desirable. NEW
methodDevelopment of a targeted GFP Reconstitution Across Synaptic Partners(GRASP) method, t-GRASP, for assessing neural connectivity is described.
resultsNumerous different pre-synaptic and post-synaptic/dendritic proteins were tested for enhancing the specificity of GRASP signal to synaptic regions. Pairing of both targeted pre- and post-t-GRASP constructs resulted in strong preferential GRASP signal in synaptic regions in Drosophila larval sensory neurons, larval neuromuscular junctions, and adult photoreceptor neurons with minimal false-positive signal. COMPARISON WITH EXISTING
methodsActivity-independent t-GRASP exhibits an enhancement of GRASP signal specificity for synaptic contact sites as compared to existing Drosophila GRASP methods. Fly strains were developed for expression of both pre- and post-t-GRASP with each of the three Drosophila binary transcription systems, thus enabling GRASP assays to be performed between any two driver pairs of any transcription system in either direction, an option not available for existing Drosophila GRASP methods.
conclusionst-GRASP is a novel targeted GRASP method for assessing synaptic connectivity between Drosophila neurons. Its flexibility of use with all three Drosophila binary transcription systems significantly expands the potential use of GRASP in Drosophila.
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