ArticleJournal of translational medicine2018
Wedge-shaped microfluidic chip for circulating tumor cells isolation and its clinical significance in gastric cancer.
Article in Journal of translational medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Liquid biopsy biomarkers for early detection of gastrointestinal cancers: Current landscape and emerging technologies.Clinical and translational medicine · 2026Review
- Modeling of Tumor Cell Adhesion, Extravasation, and Sorting in a Microvascular System.Advances in experimental medicine and biology · 2026Article
- Review
- Advances and potentials in platelet-circulating tumor cell crosstalk.American journal of cancer research · 2025Review
- The Applications and Challenges of the Development ofCellular and molecular bioengineering · 2023Review
- Impact of endoscopic metallic stent placement and emergency surgery on detection of viable circulating tumor cells for acute malignant left-sided colonic obstruction.World journal of surgical oncology · 2023Article
- Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells.Biosensors · 2022Review
- Circulating Tumor Cell Identification Based on Deep Learning.Frontiers in oncology · 2022Article
- EMT-cancer cells-derived exosomal miR-27b-3p promotes circulating tumour cells-mediated metastasis by modulating vascular permeability in colorectal cancer.Clinical and translational medicine · 2021Article
- Circulating Tumor Cells and TWIST Expression in Patients with Metastatic Gastric Cancer: A Preliminary Study.Journal of clinical medicine · 2021Article
- System Modularity Chip for Analysis of Rare Targets (SMART-Chip): Liquid Biopsy Samples.ACS sensors · 2021Article
- Clinical Applications of Liquid Biopsy in Gastric Cancer.Frontiers in medicine · 2021Review
- Negative depletion mediated brightfield circulating tumour cell identification strategy on microparticle-based microfluidic chip.Journal of nanobiotechnology · 2020Article
- Clinical Significance of Circulating Tumor Cells in Gastrointestinal Carcinomas.Diagnostics (Basel, Switzerland) · 2020Review
- Review
- Cell Separations and Sorting.Analytical chemistry · 2020Review
- A New Method for CTC Images Recognition Based on Machine Learning.Frontiers in bioengineering and biotechnology · 2020Article
- The Pharmacological Effects of Spatholobi Caulis Tannin in Cervical Cancer and Its Precise Therapeutic Effect on Related circRNA.Molecular therapy oncolytics · 2019Article
- Microfluidics for studying metastatic patterns of lung cancer.Journal of nanobiotechnology · 2019Review
- Crosstalk between cancer cells and tumor associated macrophages is required for mesenchymal circulating tumor cell-mediated colorectal cancer metastasis.Molecular cancer · 2019Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundCirculating tumor cells (CTCs) have great potential in both basic research and clinical application for the managements of cancer. However, the complicated fabrication processes and expensive materials of the existing CTCs isolation devices, to a large extent, limit their clinical translation and CTCs' clinical value. Therefore, it remains to be urgently needed to develop a new platform for achieving CTCs detection with low-cost, mass-producible but high performance.
methodsIn the present study, we introduced a novel wedge-shaped microfluidic chip (named CTC-ΔChip) fabricated by two pieces of glass through wet etching and thermal bonding technique for CTCs isolation, which achieved CTCs enrichment by different size without cell surface expression markers and CTCs identification with three-color immunocytochemistry method (CK+/CD45-/Nucleus+). We validated the feasibility of CTC-ΔChip for detecting CTCs from different types of solid tumor. Furthermore, we applied the newly-developed platform to investigate the clinical significance of CTCs in gastric cancer (GC).
resultsBased on "label-free" characteristic, the capture efficiency of CTC-ΔChip can be as high as 93.7 ± 3.2% in DMEM and 91.0 ± 3.0% in whole blood sample under optimized conditions. Clinically, CTC-ΔChip exhibited the feasibility of detecting CTCs from different types of solid tumor, and it identified 7.30 ± 7.29 CTCs from 2 mL peripheral blood with a positive rate of 75% (30/40) in GC patients. Interestingly, we found that GC CTCs count was significantly correlated with multiple systemic inflammation indexes, including the lymphocyte count, platelet count, the level of neutrophil to lymphocyte ratio and platelet to lymphocyte ratio. In addition, we also found that both the positivity rate and CTCs count were significantly associated with multiple clinicopathology parameters.
conclusionsOur novel CTC-ΔChip shows high performance for detecting CTCs from less volume of blood samples of cancer patients and important clinical significance in GC. Owing to the advantages of low-cost and mass-producible, CTC-ΔChip holds great potential of clinical application for cancer therapeutic guidance and prognostic monitoring in the future.
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