Evidence map›Paper›PMID 29791489›Full record

ArticlePloS one2018

Contractile force measurement of human induced pluripotent stem cell-derived cardiac cell sheet-tissue.

Daisuke Sasaki, Katsuhisa Matsuura, Hiroyoshi Seta, Yuji Haraguchi, Teruo Okano, Tatsuya Shimizu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Daisuke SasakiInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
Katsuhisa MatsuuraInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
Hiroyoshi SetaDepartment of Cardiovascular Surgery, Tokyo Women's Medical University, Tokyo, Japan.
Yuji HaraguchiInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
Teruo OkanoInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
Tatsuya ShimizuInstitute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.ORCID 0000-0001-7601-4239

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have developed our original tissue engineering technology "cell sheet engineering" utilizing temperature-responsive culture dishes. The cells are confluently grown on a temperature-responsive culture dish and can be harvested as a cell sheet by lowering temperature without enzymatic digestion. Cell sheets are high-cell-density tissues similar to actual living tissues, maintaining their structure and function. Based on this "cell sheet engineering", we are trying to create functional cardiac tissues from human induced pluripotent stem cells, for regenerative therapy and in vitro drug testing. Toward this purpose, it is necessary to evaluate the contractility of engineered cardiac cell sheets. Therefore, in the present study, we developed a contractile force measurement system and evaluated the contractility of human iPSC-derived cardiac cell sheet-tissues. By attaching the cardiac cell sheets on fibrin gel sheets, we created dynamically beating cardiac cell sheet-tissues. They were mounted to the force measurement system and the contractile force was measured stably and clearly. The absolute values of contractile force were around 1 mN, and the mean force value per cross-sectional area was 3.3 mN/mm2. These values are equivalent to or larger than many previously reported values, indicating the functionality of our engineered cardiac cell sheets. We also confirmed that both the contractile force and beating rate were significantly increased by the administration of adrenaline, which are the physiologically relevant responses for cardiac tissues. In conclusion, the force measurement system developed in the present study is valuable for the evaluation of engineered cardiac cell sheet-tissues, and for in vitro drug testing as well.

Indexed as

Muscle ContractionAnimalsDrug Evaluation, PreclinicalEpinephrineHumansInduced Pluripotent Stem CellsMyocytes, CardiacTissue EngineeringEpinephrine

Identifiers

PMID29791489
PMCPMC5965888

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.