ArticleHistochemistry and cell biology2018
Precise and economic FIB/SEM for CLEM: with 2 nm voxels through mitosis.
Article in Histochemistry and cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- From Microscopy to Nanoscopy: Contemporary Physical Methods in Mitochondrial Structural Biology.International journal of molecular sciences · 2026Review
- Cryo-SEM and large volume FIB-SEM of Arabidopsis cotyledons: Degradation of lipid bodies, biogenesis of glyoxysomes and reorganisation of organelles during germination.Journal of microscopy · 2025Article
- CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.The Journal of cell biology · 2023Article
- Evidence of a role for cAMP in mitochondrial regulation in ovarian granulosa cells.Molecular human reproduction · 2022Article
- Changes in Envelope Structure and Cell-Cell Communication during Akinete Differentiation and Germination in Filamentous CyanobacteriumLife (Basel, Switzerland) · 2022Article
- An RNAi screen for genes that affect nuclear morphology in Caenorhabditis elegans reveals the involvement of unexpected processes.G3 (Bethesda, Md.) · 2021Article
- Inheritance of the reduced mitochondria of Giardia intestinalis is coupled to the flagellar maturation cycle.BMC biology · 2021Article
- New Insights Into Sperm Ultrastructure Through Enhanced Scanning Electron Microscopy.Frontiers in cell and developmental biology · 2021Article
- In focus in HCB.Histochemistry and cell biology · 2020Article
- C. elegans pronuclei fuse after fertilization through a novel membrane structure.The Journal of cell biology · 2020Article
- Insights into replicative senescence of human testicular peritubular cells.Scientific reports · 2019Article
- In focus in HCB.Histochemistry and cell biology · 2019Article
- Gold-substituted Silver-intensified Peroxidase Immunolabeling for FIB-SEM Imaging.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2019Article
- From Light Microscopy to Analytical Scanning Electron Microscopy (SEM) and Focused Ion Beam (FIB)/SEM in Biology: Fixed Coordinates, Flat Embedding, Absolute References.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2018Article
- Label-free 3D-CLEM Using Endogenous Tissue Landmarks.iScience · 2018Article
- In focus in HCB.Histochemistry and cell biology · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A portfolio is presented documenting economic, high-resolution correlative focused ion beam scanning electron microscopy (FIB/SEM) in routine, comprising: (i) the use of custom-labeled slides and coverslips, (ii) embedding of cells in thin, or ultra-thin resin layers for correlative light and electron microscopy (CLEM) and (iii) the claim to reach the highest resolution possible with FIB/SEM in xyz. Regions of interest (ROIs) defined in light microscope (LM), can be relocated quickly and precisely in SEM. As proof of principle, HeLa cells were investigated in 3D context at all stages of the cell cycle, documenting ultrastructural changes during mitosis: nuclear envelope breakdown and reassembly, Golgi degradation and reconstitution and the formation of the midzone and midbody.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.