ArticleScientific reports2018
Microbial production of novel sulphated alkaloids for drug discovery.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 61 citations in OpenAlex.
- Benzylisoquinoline Alkaloid Production in Yeast via Norlaudanosoline Improves Titer, Selectivity, and Yield.ACS synthetic biology · 2026Article
- Biosynthetic Strategies of Berberine Bridge Enzyme-like Flavoprotein Oxidases toward Structural Diversification in Natural Product Biosynthesis.Biochemistry · 2024Review
- A CRISPR-Cas9-Mediated Large-Fragment Assembly Method for Cloning Genomes and Biosynthetic Gene Cluster.Microorganisms · 2024Article
- An Enzymatic Strategy for the Selective Methylation of High-Value-Added Tetrahydroprotoberberine Alkaloids.International journal of molecular sciences · 2023Article
- Engineered Escherichia coli platforms for tyrosine-derivative production from phenylalanine using phenylalanine hydroxylase and tetrahydrobiopterin-regeneration system.Biotechnology for biofuels and bioproducts · 2023Article
- Multi-Level Optimization and Strategies in Microbial Biotransformation of Nature Products.Molecules (Basel, Switzerland) · 2023Review
- Metabolic engineering for the biosynthesis of bis-indolylquinone terrequinone A in Escherichia coli from L-tryptophan and prenol.Biotechnology for biofuels and bioproducts · 2023Article
- From Steroid and Drug Metabolism to Glycobiology, Using Sulfotransferase Structures to Understand and Tailor Function.Drug metabolism and disposition: the biological fate of chemicals · 2022Review
- Biotechnological production of specialty aromatic and aromatic-derivative compounds.World journal of microbiology & biotechnology · 2022Review
- Machine learning discovery of missing links that mediate alternative branches to plant alkaloids.Nature communications · 2022Article
- Transport engineering for improving the production and secretion of valuable alkaloids inMetabolic engineering communications · 2021Article
- Establishment of a co-culture system using Escherichia coli and Pichia pastoris (Komagataella phaffii) for valuable alkaloid production.Microbial cell factories · 2021Article
- EngineeringaBIOTECH · 2021Review
- Biosynthesis and Biological Activities of Newly Discovered Amaryllidaceae Alkaloids.Molecules (Basel, Switzerland) · 2020Review
- Back to the plant: overcoming roadblocks to the microbial production of pharmaceutically important plant natural products.Journal of industrial microbiology & biotechnology · 2020Review
- A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids.Nature communications · 2020Article
- Recent Advances in Metabolically Engineered Microorganisms for the Production of Aromatic Chemicals Derived From Aromatic Amino Acids.Frontiers in bioengineering and biotechnology · 2020Review
- Production of zosteric acid and other sulfated phenolic biochemicals in microbial cell factories.Nature communications · 2019Article
- Sulfur-Containing Carotenoids from A Marine Coral SymbiontMarine drugs · 2019Article
- Synthetic biology strategies for microbial biosynthesis of plant natural products.Nature communications · 2019Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natural products from plants are useful as lead compounds in drug discovery. Plant benzylisoquinoline alkaloids (BIAs) exhibit various pharmaceutical activities. Although unidentified BIAs are expected to be of medicinal value, sufficient quantities of such BIAs, for biological assays, are sometimes difficult to obtain due to their low content in natural sources. Here, we showed that high productivity of BIAs in engineered Escherichia coli could be exploited for drug discovery. First, we improved upon the previous microbial production system producing (S)-reticuline, an important BIA intermediate, to obtain yields of around 160 mg/L, which was 4-fold higher than those of the previously reported highest production system. Subsequently, we synthesised non-natural BIAs (O-sulphated (S)-reticulines) by introducing human sulphotransferases into the improved (S)-reticuline production system. Analysis of human primary cells treated with these BIAs demonstrated that they affected a biomarker expression in a manner different from that by the parent compound (S)-reticuline, suggesting that simple side-chain modification altered the characteristic traits of BIA. These results indicated that highly productive microbial systems might facilitate the production of scarce or novel BIAs and enable subsequent evaluation of their biological activities. The system developed here could be applied to other rare natural products and might contribute to the drug-discovery process as a next-generation strategy.
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