ArticleNature communications2018
Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed.
- Advances in large DNA fragment assembly for microbial cell factory engineering.Quantitative biology (Beijing, China) · 2026Review
- Synthetic rewriting of the IGH locus.Cell genomics · 2026Article
- Synthetic rewriting technologies in mammalian cells.Nature communications · 2026Review
- Yeast strain development and process intensification in high-gravity fermentation.FEMS yeast research · 2026Review
- EASY-C: Extraction and Analysis of Small Yeast Chromosomes-A rapid and universal platform for recovering artificial mini-chromosomes from synthetic Sc2.0 yeast and large plasmids fromSynthetic biology (Oxford, England) · 2026Article
- Iterative SCRaMbLE for engineering synthetic genome modules and chromosomes.Nature communications · 2025Article
- The design and engineering of synthetic genomes.Nature reviews. Genetics · 2025Review
- Synthetic evolution ofmLife · 2025Review
- Evolutionary engineering ofScience advances · 2024Article
- Laccase is a multitasking protein for synthetic gene circuits in the yeastSynthetic and systems biotechnology · 2024Article
- Dual genetic level modification engineering accelerate genome evolution of Corynebacterium glutamicum.Nucleic acids research · 2024Article
- An Optimized Genotyping Workflow for Identifying Highly SCRaMbLEd Synthetic Yeasts.ACS synthetic biology · 2024Article
- Genome-Wide Identification, Expression Analysis under Abiotic Stress and Co-Expression Analysis ofInternational journal of molecular sciences · 2024Article
- Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae.Nature communications · 2024Article
- Genome-Wide Screen for Enhanced Noncanonical Amino Acid Incorporation in Yeast.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Dissecting aneuploidy phenotypes by constructing Sc2.0 chromosome VII and SCRaMbLEing synthetic disomic yeast.Cell genomics · 2023Article
- Synthetic yeast chromosome XI design provides a testbed for the study of extrachromosomal circular DNA dynamics.Cell genomics · 2023Article
- Practical Approaches for the YeastInternational journal of molecular sciences · 2023Review
- A self-propagating, barcoded transposon system for the dynamic rewiring of genomic networks.Molecular systems biology · 2023Article
- Dynamics of synthetic yeast chromosome evolution shaped by hierarchical chromatin organization.National science review · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SCRaMbLE is a novel system implemented in the synthetic yeast genome, enabling massive chromosome rearrangements to produce strains with a large genotypic diversity upon induction. Here we describe a reporter of SCRaMbLEd cells using efficient selection, termed ReSCuES, based on a loxP-mediated switch of two auxotrophic markers. We show that all randomly isolated clones contained rearrangements within the synthetic chromosome, demonstrating high efficiency of selection. Using ReSCuES, we illustrate the ability of SCRaMbLE to generate strains with increased tolerance to several stress factors, such as ethanol, heat and acetic acid. Furthermore, by analyzing the tolerant strains, we are able to identify ACE2, a transcription factor required for septum destruction after cytokinesis, as a negative regulator of ethanol tolerance. Collectively, this work not only establishes a generic platform to rapidly identify strains of interest by SCRaMbLE, but also provides methods to dissect the underlying mechanisms of resistance.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.