ArticleNucleic acids research2018
Cooperative binding of ApiAP2 transcription factors is crucial for the expression of virulence genes in Toxoplasma gondii.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- A Live Attenuated AP2X-1-DeficientAnimals : an open access journal from MDPI · 2026Article
- A guide to the apicomplexan cell cycle: endodyogeny and schizogony.Trends in parasitology · 2025Review
- Deletion of splicing factor Cdc5 in Toxoplasma disrupts transcriptome integrity, induces abortive bradyzoite formation, and prevents acute infection in mice.Nature communications · 2025Article
- A Novel Nuclear Protein Complex Controlling the Expression of Developmentally Regulated Genes in Toxoplasma Gondii.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Article
- Cascading expression of ApiAP2 transcription factors controls daughter cell assembly in Toxoplasma gondii.PLoS pathogens · 2024Article
- Identification and function characterization of NcAP2XII-4 in Neospora caninum.Parasites & vectors · 2024Article
- Single cell expression and chromatin accessibility of the Toxoplasma gondii lytic cycle identifies AP2XII-8 as an essential ribosome regulon driver.Nature communications · 2024Article
- Article
- Article
- AP2XII-1 and AP2XI-2 Suppress Schizogony Gene Expression inInternational journal of molecular sciences · 2024Article
- The multifaceted roles of Myb domain-containing proteins in apicomplexan parasites.Current opinion in microbiology · 2023Review
- Cell cycle-regulated ApiAP2s and parasite development: the Toxoplasma paradigm.Current opinion in microbiology · 2023Review
- Comparative single-cell transcriptional atlases of Babesia species reveal conserved and species-specific expression profiles.PLoS biology · 2022Article
- Nuclear Factor AP2X-4 Governs the Expression of Cell Cycle- and Life Stage-Regulated Genes and is Critical forMicrobiology spectrum · 2022Article
- A toolbox for conditional control of gene expression in apicomplexan parasites.Molecular microbiology · 2022Review
- RegEnrich gene regulator enrichment analysis reveals a key role of the ETS transcription factor family in interferon signaling.Communications biology · 2022Article
- Primary brain cell infection byOpen biology · 2021Article
- TgAP2IX-5 is a key transcriptional regulator of the asexual cell cycle division in Toxoplasma gondii.Nature communications · 2021Article
- Dynamically expressed genes provide candidate viability biomarkers in a model coccidian.PloS one · 2021Article
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Authors and funding
6 authors.
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Abstract
Toxoplasma gondii virulence depends on the expression of factors packed into specific organelles such as rhoptry and microneme. Although virulence factor expression is tightly regulated, the molecular mechanisms controlling their regulation remain poorly understood. ApiAP2 are a family of conserved transcription factors (TFs) that play an important role in regulating gene expression in apicomplexan parasites. TgAP2XI-5 is able to bind to transcriptionally active promoters of genes expressed during the S/M phase of the cell cycle, such as virulence genes (rhoptries and micronemes genes). We identified proteins interacting with TgAP2XI-5 including a cell cycle-regulated ApiAP2 TF, TgAP2X-5. Using an inducible knock-down strategy and RNA-seq, we demonstrated that the level of expression of number of virulence factors transcripts is affected by the disruption of TgAP2X-5 expression. While TgAP2X-5 disruption has mild effect on parasite invasion, it leads to the strain avirulence in mice. To better understand the molecular mechanisms at stake, we investigated the binding of TgAP2XI-5 at promoters in the TgAP2X-5 mutant strain in a genome-wide assay. We show that disruption of TgAP2X-5 expression leads to defects in TgAP2XI-5 binding to multiple rhoptry gene promoters. Taken together, these data suggest a cooperative contribution of two ApiAP2 TF in the regulation of virulence genes in T. gondii.
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