ArticlePloS one2018
Evaluation of anaesthetic protocols for laboratory adult zebrafish (Danio rerio).
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 78 citations in OpenAlex.
- Twenty-four-hour post-anaesthetic behavioural effects of menthol and thymol compared to conventional anaesthetics in zebrafish.Fish physiology and biochemistry · 2026Article
- Novel insights into eugenol anaesthesia in poeciliid larvae: Preliminary study of gill histology.Journal of fish biology · 2026Article
- Evaluation of thymol application for anaesthesia of adult zebrafish.Fish physiology and biochemistry · 2025Article
- Menthol as a sustainable alternative anaesthetic for adult zebrafish (Danio rerio).Veterinary research communications · 2025Article
- Nafamostat Mesylate Regulates Glycosylation to Alleviate Aristolochic Acid Induced Kidney Injury.Toxins · 2025Article
- Zebrafish identification with deep CNN and ViT architectures using a rolling training window.Scientific reports · 2025Article
- Tricaine, eugenol and etomidate for repetitive procedural anesthesia in adult zebrafish,Frontiers in veterinary science · 2025Article
- Evaluation of the Effectiveness of Eugenol and MS-222 as Anesthetics in Zebrafish in Repeated Exposures and Post-Anesthesia Behaviour.Animals : an open access journal from MDPI · 2024Article
- Assessment of the effect of tricaine (MS-222)-induced anesthesia on brain-wide neuronal activity of zebrafish (Frontiers in neuroscience · 2024Article
- Behavioural Aversion and Cortisol Level Assessment When Adult Zebrafish Are Exposed to Different Anaesthetics.Biology · 2022Article
- Glutamate NMDA Receptor Antagonists with Relevance to Schizophrenia: A Review of Zebrafish Behavioral Studies.Current neuropharmacology · 2022Review
- Embryonic exposure to fentanyl induces behavioral changes and neurotoxicity in zebrafish larvae.PeerJ · 2022Article
- Screening of Anaesthetics in Adult Zebrafish (Biology · 2021Article
- Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish.Disease models & mechanisms · 2021Article
- Apex Resection in Zebrafish (International journal of molecular sciences · 2021Article
- Identification of Individual Zebrafish (Animals : an open access journal from MDPI · 2021Article
- Elevated preoptic brain activity in zebrafish glial glycine transporter mutants is linked to lethargy-like behaviors and delayed emergence from anesthesia.Scientific reports · 2021Article
- A suitable anaesthetic protocol for metamorphic zebrafish.PloS one · 2021Article
- A Comparison of Buffered Tricaine Methanesulfonate (MS-222) and Isoeugenol Anesthesia for Caudal Fin Clipping in Zebrafish (Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- A comparison between chemical and gas hypoxia as models of global ischemia in zebrafish (Animal models and experimental medicine · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the last decades, the use of zebrafish (Danio rerio) in biomedical research has increased. Anaesthesia is daily used in fish during experimental procedures to avoid discomfort, stress or pain. Also, fish welfare and the reliability of results can be compromised if an unsuitable anaesthetic protocol is used. Therefore, we aimed to refine anaesthetic protocols to be used in adult zebrafish by evaluating the efficacy of different anaesthetics, used alone or in combination. For that, zebrafish were randomly assigned to 8 different groups: 100 μg/mLMS-222 (MS); 0.2 μg/mL etomidate (E); 0.2 μg/mL etomidate + 100 μg/mL lidocaine (E+L); 1.25 μg/mL propofol (P); 1.25 μg/mL propofol + 100 μg/mL lidocaine (P+L); 100 μg/mL ketamine (K); 100 μg/mL ketamine + 1.25 μg/mL medetomidine (K+M); and 100 μg/mL ketamine + 1.25 μg/mL medetomidine/3.125 μg/mL atipamezole (K+M/A). The animals were placed in an anaesthetic water bath, then, the following parameters were registered: time for equilibrium loss and anaesthesia induction, loss of sensitivity to soft and painful stimuli, respiratory rate, recovery time, and activity after recovery. The combined forms of E+L, P+L and K+M were the fastest to induce a surgical anaesthetic stage. Nevertheless, E+L induced respiratory depression, while K+M was shown to have the longer recovery time compared to MS-222, even when atipamezole was added. In conclusion, the P+L combination was shown to provide good anaesthesia with analgesia, without causing a major respiratory depression, providing as well a quick recovery, similar to MS-222.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.