Evidence map›Paper›PMID 29785618›Full record

ArticleParasitology research2018

A quick and simple benchtop vortex egg-disruption approach for the molecular diagnosis of Fasciola hepatica from ruminant faecal samples.

Nichola Eliza Davies Calvani, Tina Cheng, Christine Green, Patrick Hughes, Emily Kwan, Elizabeth Maher, Russell David Bush, Jan Šlapeta

Abstract read
PubMed Publisher
In one paragraph

Article in Parasitology research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nichola Eliza Davies CalvaniLaboratory of Veterinary Parasitology, Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camperdown, NSW, Australia.
Tina ChengLaboratory of Veterinary Parasitology, Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camperdown, NSW, Australia.
Christine GreenLaboratory of Veterinary Parasitology, Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camperdown, NSW, Australia.
Patrick HughesLaboratory of Veterinary Parasitology, Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camperdown, NSW, Australia.
Emily KwanLaboratory of Veterinary Parasitology, Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camperdown, NSW, Australia.
Elizabeth MaherLaboratory of Veterinary Parasitology, Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camperdown, NSW, Australia.
Russell David BushMekong Livestock Research Group, Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camperdown, NSW, Australia.
Jan ŠlapetaLaboratory of Veterinary Parasitology, Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camperdown, NSW, Australia. jan.slapeta@sydney.edu.au.ORCID http://orcid.org/0000-0003-1459-9117

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Commonly employed diagnostic methods for Fasciola spp., such as a traditional sedimentation and faecal egg count, or a commercially available coprological ELISA, have limitations in their sensitivity or ability to differentiate species. A reliable DNA isolation method coupled with real-time PCR addresses these issues by providing highly sensitive and quantitative molecular diagnosis from faecal samples. The current study evaluated a standard benchtop vortex for F. hepatica egg disruption in sheep and cattle faecal samples and determined the minimum faecal egg load required for a positive result from un-concentrated (raw) faecal samples. The minimum faecal egg load for a positive real-time PCR result from 150 mg raw faecal sample was 10 and 20 eggs per gram for sheep and cattle, respectively. No significant difference (P = 0.4467) between disruptions on a benchtop vortex for 5 or 10 min was observed when compared to 40 s of disruption at 6.0 m/s in a benchtop homogeniser.

Indexed as

AnimalsCattleCattle DiseasesDNA, HelminthFasciola hepaticaFascioliasisFecesFemaleParasite Egg CountReal-Time Polymerase Chain ReactionSensitivity and SpecificitySheepSheep DiseasesDNA, HelminthDNA isolationFasciolosisMolecular diagnosticsReal-time PCRVortex

Identifiers

PMID29785618

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.