ArticleLaboratory investigation; a journal of technical methods and pathology2018
Hyaluronan negatively regulates vascular calcification involving BMP2 signaling.
Article in Laboratory investigation; a journal of technical methods and pathology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- PDZ domain containing protein 1 aggravates mouse vascular smooth muscle cells calcification via PDZ1 domain mediated β-catenin stabilization and nuclear translocation.Communications biology · 2025Article
- Predictive nomogram model for severe coronary artery calcification in end-stage kidney disease patients.Renal failure · 2024Article
- Phosphoglycerate Dehydrogenase Overexpression Inhibits Ferroptosis to Repress Calcification of Human Coronary Artery Vascular Smooth Muscle Cells via the P53/SLC7A11 Pathway.International journal of general medicine · 2024Article
- CD163+ macrophages restrain vascular calcification, promoting the development of high-risk plaque.JCI insight · 2023Article
- Role of inflammation and immunity in vascular calcification: a bibliometric and visual analysis, 2000-2022.Frontiers in cardiovascular medicine · 2023Article
- Statins attenuate cholesterol-induced ROS via inhibiting NOX2/NOX4 and mitochondrial pathway in collecting ducts of the kidney.BMC nephrology · 2022Article
- Hydrogen sulfide prevents arterial medial calcification in rats with diabetic nephropathy.BMC cardiovascular disorders · 2021Article
- Role of Glycosylation in Vascular Calcification.International journal of molecular sciences · 2021Review
- Spermidine inhibits vascular calcification in chronic kidney disease through modulation of SIRT1 signaling pathway.Aging cell · 2021Article
- Tubular Mas receptor mediates lipid-induced kidney injury.Cell death & disease · 2021Article
- Bibliometric and Visual Analysis of Vascular Calcification Research.Frontiers in pharmacology · 2021Article
- Positive Association of Leptin and Artery Calcification of Lower Extremity in Patients With Type 2 Diabetes Mellitus: A Pilot Study.Frontiers in endocrinology · 2021Article
- High-Density Mineralized Protrusions and Central Osteophytes: Associated Osteochondral Junction Abnormalities in Osteoarthritis.Diagnostics (Basel, Switzerland) · 2020Article
- Bearing My Heart: The Role of Extracellular Matrix on Cardiac Development, Homeostasis, and Injury Response.Frontiers in cell and developmental biology · 2020Review
- The pathophysiological basis of vascular disease.Laboratory investigation; a journal of technical methods and pathology · 2019Article
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14 authors.
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Abstract
Vascular calcification is a highly regulated biological process similar to bone formation involving osteogenic differentiation of vascular smooth muscle cells (VSMCs). Hyaluronan (HA), a major structural component of the extracellular matrix in cartilage, has been shown to inhibit osteoblast differentiation. However, whether HA affects osteogenic differentiation and calcification of VSMCs remains unclear. In the present study, we used in vitro and ex vivo models of vascular calcification to investigate the role of HA in vascular calcification. Both high and low molecular weight HA treatment significantly reduced calcification of rat VSMCs in a dose-dependent manner, as detected by alizarin red staining and calcium content assay. Ex vivo study further confirmed the inhibitory effect of HA on vascular calcification. Similarly, HA treatment decreased ALP activity and expression of bone-related molecules including Runx2, BMP2 and Msx2. By contrast, inhibition of HA synthesis by 4-methylumbelliferone (4MU) promoted calcification of rat VSMCs. In addition, adenovirus-mediated overexpression of HA synthase 2 (HAS2), a major HA synthase in VSMCs, also inhibited calcification of VSMCs, whereas CRISPR/Cas9-mediated HAS2 knockout promoted calcification of rat A10 cells. Furthermore, we found that BMP2 signaling was inhibited in VSMCs after HA treatment. Recombinant BMP2 enhanced high calcium and phosphate-induced VSMC calcification, which can be blocked by HA treatment. Taken together, these findings suggest that HA inhibits vascular calcification involving BMP2 signaling.
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