ArticleScientific reports2018
The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
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- Using Beads as a Focus Fiduciary to Aid Software-Based Autofocus Accuracy in Microscopy.Bio-protocol · 2025Article
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- A chemical-genetic system to rapidly inhibit the PP2A-B56 phosphatase reveals a role at metaphase kinetochores.Nature communications · 2025Article
- Quantitative Live-Cell Imaging to Study Chromatin Segregation and Nuclear Reformation.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Monitoring nucleolar-nucleoplasmic protein shuttling in living cells by high-content microscopy and automated image analysis.Nucleic acids research · 2024Article
- From feulgen to modern methods: marking a century of DNA imaging advances.Histochemistry and cell biology · 2024Review
- Perspective on quantitative phase imaging to improve precision cancer medicine.Journal of biomedical optics · 2024Article
- Calreticulin and JAK2V617F driver mutations induce distinct mitotic defects in myeloproliferative neoplasms.Scientific reports · 2024Article
- Loss-of-function mitochondrial DNA polymerase gamma variants cause vascular smooth muscle cells to secrete a diffusible mitogenic factor.Frontiers in physiology · 2024Article
- SiR-DNA/SiR-Hoechst-induced chromosome entanglement generates severe anaphase bridges and DNA damage.Life science alliance · 2023Article
- A bifunctional kinase-phosphatase module balances mitotic checkpoint strength and kinetochore-microtubule attachment stability.The EMBO journal · 2023Article
- Label-free microscopy for virus infections.Microscopy (Oxford, England) · 2023Article
- OGG1 competitive inhibitors show important off-target effects by directly inhibiting efflux pumps and disturbing mitotic progression.Frontiers in cell and developmental biology · 2023Article
- A synergistic strategy to develop photostable and bright dyes with long Stokes shift for nanoscopy.Nature communications · 2022Article
- HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein.Nucleic acids research · 2022Article
- The Aurora B gradient sustains kinetochore stability in anaphase.Cell reports · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10-25 µM. However, measuring nuclear import of Cyclin B1, inhibition of mitotic entry, and the induction of γH2AX foci in cultured human cells reveals that SiR-Hoechst induces DNA damage responses and G2 arrest at concentrations well below 1 µM. SiR-Hoechst is useful for live cell imaging, but it should be used with caution and at the lowest practicable concentration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.