Evidence map›Paper›PMID 29784937›Full record

ArticleScientific reports2018

An Oil-Free Picodrop Bioassay Platform for Synthetic Biology.

Christian A Siltanen, Russell H Cole, Sean Poust, Lawrence Chao, Jabus Tyerman, Benjamin Kaufmann-Malaga, Jeff Ubersax, Zev J Gartner, Adam R Abate

Abstract read
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Droplet Microfluidics for Microbial Biotechnology.Advances in biochemical engineering/biotechnology · 2022
    Article
  6. Article
  7. Review
  8. Article
  9. Review
  10. Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Christian A SiltanenDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
Russell H ColeDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
Sean PoustDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
Lawrence ChaoAmyris, Inc. Emeryville, California, USA.
Jabus TyermanAmyris, Inc. Emeryville, California, USA.
Benjamin Kaufmann-MalagaAmyris, Inc. Emeryville, California, USA.
Jeff UbersaxAmyris, Inc. Emeryville, California, USA.
Zev J GartnerDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA.
Adam R AbateDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA. arabate@gmail.com.

Funding

Gigapixel digital PCR in Giant Unilamellar VesiclesR01EB019453 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ABATE, ADAM R., CHIU, CHARLES YEN · 2014 to 2022
$3.9M
Microfluidic immunoprofiling for biomarker discovery in rheumatoid arthritisDP2AR068129 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ABATE, ADAM R. · 2014 to 2014
$2.4M
Accurate and unbiased single cell sequencing with digital droplet MDAR01HG008978 · NHGRI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ABATE, ADAM R. · 2016 to 2018
$1.9M
SINGLE-CELL TRANSCRIPTOMIC ANALYSIS OF HIV-INFECTED CD4 T CELLS IN BLOOD AND LYMPHOID TISSUE USING PCR-ACTIVATED CELL SORTINGU01AI129206 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ABATE, ADAM R., BORITZ, ELI A · 2017 to 2020
$1.6M
Accurate, long-read sequencing with droplet-microfluidic barcodingR21HG007233 · NHGRI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ABATE, ADAM R., HERNANDEZ, RYAN D. · 2013 to 2014
$433k
NHGRI NIH HHS R01 HG008978NHGRI NIH HHS R21 HG007233NIAID NIH HHS U01 AI129206NIAMS NIH HHS DP2 AR068129NIBIB NIH HHS R01 EB019453
6 · The paper itself

Abstract

Droplet microfluidics enables massively-parallel analysis of single cells, biomolecules, and chemicals, making it valuable for high-throughput screens. However, many hydrophobic analytes are soluble in carrier oils, preventing their quantitative analysis with the method. We apply Printed Droplet Microfluidics to construct defined reactions with chemicals and cells incubated under air on an open array. The method interfaces with most bioanalytical tools and retains hydrophobic compounds in compartmentalized reactors, allowing their quantitation.

Indexed as

Synthetic BiologyBiological AssayMicrofluidic Analytical TechniquesOilsPrinting, Three-DimensionalSaccharomyces cerevisiaeSesquiterpenesbeta-farneseneOilsSesquiterpenes

Identifiers

PMID29784937
PMCPMC5962535

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.