Evidence map›Paper›PMID 29783662›Full record

ReviewSensors (Basel, Switzerland)2018

SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics.

Anna Florinskaya, Pavel Ershov, Yuri Mezentsev, Leonid Kaluzhskiy, Evgeniy Yablokov, Alexei Medvedev, Alexis Ivanov

Open access · goldAbstract readReview
In one paragraph

Review in Sensors (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.2field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
  2. Article
  3. Protein Interaction Analysis by Surface Plasmon Resonance.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  4. Article
  5. Review
  6. Review
  7. Review
  8. Peptide Affinity Chromatography Applied to Therapeutic Antibodies Purification.International journal of peptide research and therapeutics · 2021
    Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Anna FlorinskayaInstitute of Biomedical Chemistry, 119121 Moscow, Russia. aflorin@bk.ru.ORCID 0000-0002-5677-2355
Pavel ErshovInstitute of Biomedical Chemistry, 119121 Moscow, Russia. pavel79@inbox.ru.
Yuri MezentsevInstitute of Biomedical Chemistry, 119121 Moscow, Russia. yu.mezentsev@gmail.com.
Leonid KaluzhskiyInstitute of Biomedical Chemistry, 119121 Moscow, Russia. la-kaluzhskiy@yandex.ru.ORCID 0000-0003-0979-8461
Evgeniy YablokovInstitute of Biomedical Chemistry, 119121 Moscow, Russia. evgeyablokov1988@mail.ru.
Alexei MedvedevInstitute of Biomedical Chemistry, 119121 Moscow, Russia. professor57@yandex.ru.
Alexis IvanovInstitute of Biomedical Chemistry, 119121 Moscow, Russia. alexei.ivanov@ibmc.msk.ru.
Institute of Biomedical Chemistry · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have developed an original experimental approach based on the use of surface plasmon resonance (SPR) biosensors, applicable for investigation of potential partners involved in protein⁻protein interactions (PPI) as well as protein⁻peptide or protein⁻small molecule interactions. It is based on combining a SPR biosensor, size exclusion chromatography (SEC), mass spectrometric identification of proteins (LC-MS/MS) and direct molecular fishing employing principles of affinity chromatography for isolation of potential partner proteins from the total lysate of biological samples using immobilized target proteins (or small non-peptide compounds) as ligands. Applicability of this approach has been demonstrated within the frame of the Human Proteome Project (HPP) and PPI regulation by a small non-peptide biologically active compound, isatin.

Indexed as

Biosensing TechniquesProtein Interaction MapsChromatography, GelImmobilized ProteinsLigandsMass SpectrometryProtein BindingProteinsSmall Molecule LibrariesSurface Plasmon ResonanceTandem Mass SpectrometryImmobilized ProteinsLigandsProteinsSmall Molecule Librariesdirect molecular fishingisatinLC-MS/MS protein identificationprotein interactomicssize exclusion chromatography (SEC)SPR biosensors

Identifiers

PMID29783662
PMCPMC5982148
OpenAlexW2804500936

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.