ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2018
Headspace sorptive extraction-gas chromatography-mass spectrometry method to measure volatile emissions from human airway cell cultures.
Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 40 citations in OpenAlex.
- SPME-based investigation of thapsigargin-induced alterations in the volatilome of human melanoma cells.Analytical and bioanalytical chemistry · 2026Article
- Volatile organic compounds (VOCs) to monitor cell expansion and microbial contamination of mesenchymal stromal cells (MSCs): a preliminary study.Journal of breath research · 2025Article
- An engineered culture vessel and flow system to improve the in vitro analysis of volatile organic compounds.Communications engineering · 2025Article
- Defining VOC signatures of airway epithelial cells with PM2.5 exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- Fulfilling the Promise of Breathomics: Considerations for the Discovery and Validation of Exhaled Volatile Biomarkers.American journal of respiratory and critical care medicine · 2024Review
- Discovery and Validation of a Volatile Signature of Eosinophilic Airway Inflammation in Asthma.American journal of respiratory and critical care medicine · 2024Article
- Article
- Sensor technologies for quality control in engineered tissue manufacturing.Biofabrication · 2022Review
- Breath carbonyl levels in a human population of seven hundred participants.Journal of breath research · 2020Article
- Volatile organic compound (VOC) emissions of CHO and T cells correlate to their expansion in bioreactors.Journal of breath research · 2019Article
- Modeling cellular metabolomic effects of oxidative stress impacts from hydrogen peroxide and cigarette smoke on human lung epithelial cells.Journal of breath research · 2019Article
- Thin film nanofibers containing ZnTiOMikrochimica acta · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The human respiratory tract releases volatile metabolites into exhaled breath that can be utilized for noninvasive health diagnostics. To understand the origin of this metabolic process, our group has previously analyzed the headspace above human epithelial cell cultures using solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS). In the present work, we improve our model by employing sorbent-covered magnetic stir bars for headspace sorptive extraction (HSSE). Sorbent-coated stir bar analyte recovery increased by 52 times and captured 97 more compounds than SPME. Our data show that HSSE is preferred over liquid extraction via stir bar sorptive extraction (SBSE), which failed to distinguish volatiles unique to the cell samples compared against media controls. Two different cellular media were also compared, and we found that Opti-MEM® is preferred for volatile analysis. We optimized HSSE analytical parameters such as extraction time (24 h), desorption temperature (300 °C) and desorption time (7 min). Finally, we developed an internal standard for cell culture VOC studies by introducing 842 ng of deuterated decane per 5 mL of cell medium to account for error from extraction, desorption, chromatography and detection. This improved model will serve as a platform for future metabolic cell culture studies to examine changes in epithelial VOCs caused by perturbations such as viral or bacterial infections, opening opportunities for improved, noninvasive pulmonary diagnostics.
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Registered trials
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