ArticleGastroenterology2018
Optogenetic Induction of Colonic Motility in Mice.
Article in Gastroenterology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers, 1 of them a synthesis that pooled it.
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Who cites it
56 citing papers in PubMed, 1 synthesis or guideline pooled it, 86 citations in OpenAlex.
- Optogenetic control of the enteric nervous system and gastrointestinal transit.Expert review of gastroenterology & hepatology · 2019Pooled it
- Transforming healthcare through in-body bioelectronic systems.Nature communications · 2026Review
- Neuronal regulation of myenteric interstitial cells of Cajal (ICC-MY) in the proximal colon.Cell calcium · 2025Article
- Optogenetics-integrated gut organ culture system connects enteric neurons dynamics and gut homeostasis.Nature communications · 2025Article
- Direct Stimulation of Gastric Smooth Muscle Cells via GNeurogastroenterology and motility · 2025Article
- Enteric neuronal Piezo1 maintains mechanical and immunological homeostasis by sensing force.Cell · 2025Article
- Synaptic cell adhesion moleculeeLife · 2025Article
- Article
- Unique properties of proximal and distal colon reflect distinct motor functions.American journal of physiology. Gastrointestinal and liver physiology · 2025Review
- Insights Into Gastrointestinal Motility Through the Use of Optogenetic Sensors.Journal of neurogastroenterology and motility · 2024Article
- An ingestible, battery-free, tissue-adhering robotic interface for non-invasive and chronic electrostimulation of the gut.Nature communications · 2024Article
- Enteric neural stem cell transplant restores gut motility in mice with Hirschsprung disease.JCI insight · 2024Article
- Multifunctional microelectronic fibers enable wireless modulation of gut and brain neural circuits.Nature biotechnology · 2024Article
- Systematic analysis of proximal midgut- and anorectal-originating contractions in larval zebrafish using event feature detection and supervised machine learning algorithms.Neurogastroenterology and motility · 2023Article
- Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo.Nature protocols · 2023Article
- Transplanted ENSCs form functional connections with intestinal smooth muscle and restore colonic motility in nNOS-deficient mice.Stem cell research & therapy · 2023Article
- An Optogenetics-based Approach to Regulate Colonic Contractions by Modulating the Activity of the Interstitial Cells of Cajal in Mice.Journal of neurogastroenterology and motility · 2023Article
- The enteric nervous system.Physiological reviews · 2023Review
- Wirelessly powered deformable electronic stent for noninvasive electrical stimulation of lower esophageal sphincter.Science advances · 2023Article
- Wireless Self-Powered Optogenetic System for Long-Term Cardiac Neuromodulation to Improve Post-MI Cardiac Remodeling and Malignant Arrhythmia.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
BACKGROUND &
aimsStrategies are needed to increase gastrointestinal transit without systemic pharmacologic agents. We investigated whether optogenetics, focal application of light to control enteric nervous system excitability, could be used to evoke propagating contractions and increase colonic transit in mice.
methodsWe generated transgenic mice with Cre-mediated expression of light-sensitive channelrhodopsin-2 (ChR2) in calretinin neurons (CAL-ChR2 Cre+ mice); Cre- littermates served as controls. Colonic myenteric neurons were analyzed by immunohistochemistry, patch-clamp, and calcium imaging studies. Motility was assessed by mechanical, electrophysiological, and video recording in vitro and by fecal output in vivo.
resultsIn isolated colons, focal light stimulation of calretinin enteric neurons evoked classic polarized motor reflexes (50/58 stimulations), followed by premature anterograde propagating contractions (39/58 stimulations). Light stimulation could evoke motility from sites along the entire colon. These effects were prevented by neural blockade with tetrodotoxin (n = 2), and did not occur in control mice (n = 5). Light stimulation of proximal colon increased the proportion of natural fecal pellets expelled over 15 minutes in vitro (75% ± 17% vs 32% ± 8% for controls) (P < .05). In vivo, activation of wireless light-emitting diodes implanted onto the colon wall significantly increased hourly fecal pellet output in conscious, freely moving mice (4.2 ± 0.4 vs 1.3 ± 0.3 in controls) (P < .001).
conclusionsIn studies of mice, we found that focal activation of a subset of enteric neurons can increase motility of the entire colon in vitro, and fecal output in vivo. Optogenetic control of enteric neurons might therefore be used to modify gut motility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.