ArticlePLoS neglected tropical diseases2018
Development of a toolkit for piggyBac-mediated integrative transfection of the human filarial parasite Brugia malayi.
Article in PLoS neglected tropical diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
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- CRISPR/Cas genome editing, functional genomics, and diagnostics for parasitic helminths.International journal for parasitology · 2025Review
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- Astacin metalloproteases in human-parasitic nematodes.Advances in parasitology · 2024Review
- New paradigms in research on Dirofilaria immitis.Parasites & vectors · 2023Review
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- Gene editing in the nematode parasiteFrontiers in parasitology · 2023Article
- Spatial transcriptomics reveals antiparasitic targets associated with essential behaviors in the human parasite Brugia malayi.PLoS pathogens · 2022Article
- Establishment of an in vitro culture system to study the developmental biology of Onchocerca volvulus with implications for anti-Onchocerca drug discovery and screening.PLoS neglected tropical diseases · 2021Article
- Lentiviral transduction facilitates RNA interference in the nematode parasite Nippostrongylus brasiliensis.PLoS pathogens · 2021Article
- CRISPR-mediated Transfection of Brugia malayi.PLoS neglected tropical diseases · 2020Article
- Genetic and functional diversification of chemosensory pathway receptors in mosquito-borne filarial nematodes.PLoS biology · 2020Article
- Prediction pipeline for discovery of regulatory motifs associated with Brugia malayi molting.PLoS neglected tropical diseases · 2020Article
- In vivo imaging of transgenic Brugia malayi.PLoS neglected tropical diseases · 2020Article
- Recent advances in functional genomics for parasitic nematodes of mammals.The Journal of experimental biology · 2020Review
- CRISPR/Cas9 Mutagenesis and Expression of Dominant Mutant Transgenes as Functional Genomic Approaches in Parasitic Nematodes.Frontiers in genetics · 2019Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe human filarial parasites cause diseases that are among the most important causes of morbidity in the developing world. The elimination programs targeting these infections rely on a limited number of drugs, making the identification of new chemotherapeutic agents a high priority. The study of these parasites has lagged due to the lack of reverse genetic methods. METHODOLOGY/PRINCIPAL
findingsWe report a novel co-culture method that results in developmentally competent infective larvae of one of the human filarial parasites (Brugia malayi) and describe a method to efficiently transfect the larval stages of this parasite. We describe the production of constructs that result in integrative transfection using the piggyBac transposon system, and a selectable marker that can be used to identify transgenic parasites. We describe the production and use of dual reporter plasmids containing both a secreted luciferase selectable marker and fluorescent protein reporters that will be useful to study temporal and spatial patterns of gene expression. CONCLUSIONS/SIGNIFICANCE: The methods and constructs reported here will permit the efficient production of integrated transgenic filarial parasite lines, allowing reverse genetic technologies to be applied to all life cycle stages of the parasite.
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