ArticleStem cell reports2018
Inductive and Selective Effects of GSK3 and MEK Inhibition on Nanog Heterogeneity in Embryonic Stem Cells.
Article in Stem cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Gene-specific transcript buffering revealed by perturbation of coactivator complexes.Science advances · 2025Article
- aiSEGcell: User-friendly deep learning-based segmentation of nuclei in transmitted light images.PLoS computational biology · 2024Article
- Combinatorial quantification of 5mC and 5hmC at individual CpG dyads and the transcriptome in single cells reveals modulators of DNA methylation maintenance fidelity.Nature structural & molecular biology · 2024Article
- An improved rhythmicity analysis method using Gaussian Processes detects cell-density dependent circadian oscillations in stem cells.Bioinformatics (Oxford, England) · 2023Article
- Embryonic stem cell ERK, AKT, plus STAT3 response dynamics combinatorics are heterogeneous but NANOG state independent.Stem cell reports · 2023Article
- An improved rhythmicity analysis method using Gaussian Processes detects cell-density dependent circadian oscillations in stem cells.bioRxiv : the preprint server for biology · 2023Article
- H3K9 tri-methylation at Nanog times differentiation commitment and enables the acquisition of primitive endoderm fate.Development (Cambridge, England) · 2022Article
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- Article
- Restoration of primary cilia in obese adipose-derived mesenchymal stem cells by inhibiting Aurora A or extracellular signal-regulated kinase.Stem cell research & therapy · 2019Article
- Extensive epigenetic and transcriptomic variability between genetically identical human B-lymphoblastoid cells with implications in pharmacogenomics research.Scientific reports · 2019Article
- Esrrb extinction triggers dismantling of naïve pluripotency and marks commitment to differentiation.The EMBO journal · 2018Article
- 2i, or Not 2i: The Soliloquy of Nanog-Negative Mouse Embryonic Stem Cells.Stem cell reports · 2018Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic stem cells (ESCs) display heterogeneous expression of pluripotency factors such as Nanog when cultured with serum and leukemia inhibitory factor (LIF). In contrast, dual inhibition of the signaling kinases GSK3 and MEK (2i) converts ESC cultures into a state with more uniform and high Nanog expression. However, it is so far unclear whether 2i acts through an inductive or selective mechanism. Here, we use continuous time-lapse imaging to quantify the dynamics of death, proliferation, and Nanog expression in mouse ESCs after 2i addition. We show that 2i has a dual effect: it both leads to increased cell death of Nanog low ESCs (selective effect) and induces and maintains high Nanog levels (inductive effect) in single ESCs. Genetic manipulation further showed that presence of NANOG protein is important for cell viability in 2i medium. This demonstrates complex Nanog-dependent effects of 2i treatment on ESC cultures.
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