ArticleStem cell reports2018
Identification and Single-Cell Functional Characterization of an Endodermally Biased Pluripotent Substate in Human Embryonic Stem Cells.
Article in Stem cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Oscillatory co-expression of HES1 and HES5 enables a hybrid state in a cross-repressive transcription factor regulatory motif.Development (Cambridge, England) · 2026Article
- Stem cell fate decisions: Substates and attractors.Stem cell reports · 2025Review
- Pluripotent cell states and fates in human embryo models.Development (Cambridge, England) · 2025Review
- A short history of pluripotent stem cells markers.Stem cell reports · 2024Review
- Article
- FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells.International journal of molecular sciences · 2022Article
- Cell type determination for cardiac differentiation occurs soon after seeding of human-induced pluripotent stem cells.Genome biology · 2022Article
- GATA6‑induced FN1 activation promotes the proliferation, invasion and migration of oral squamous cell carcinoma cells.Molecular medicine reports · 2022Article
- Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency.Cell stem cell · 2021Article
- Targeting a positive regulatory loop in the tumor-macrophage interaction impairs the progression of clear cell renal cell carcinoma.Cell death and differentiation · 2021Article
- Chromatin regulatory dynamics of early human small intestinal development using a directed differentiation model.Nucleic acids research · 2021Article
- Mapping germ-layer specification preventing genes in hPSCs via genome-scale CRISPR screening.iScience · 2021Article
- Unraveling the Spatiotemporal Human Pluripotency in Embryonic Development.Frontiers in cell and developmental biology · 2021Review
- Differentiation of Human Pluripotent Stem Cells Into Definitive Endoderm Cells in Various Flexible Three-Dimensional Cell Culture Systems: Possibilities and Limitations.Frontiers in cell and developmental biology · 2021Article
- Generation and trapping of a mesoderm biased state of human pluripotency.Nature communications · 2020Article
- Dynamic properties of noise and Her6 levels are optimized by miR-9, allowing the decoding of the Her6 oscillator.The EMBO journal · 2020Article
- Article
- KLF4 is involved in the organization and regulation of pluripotency-associated three-dimensional enhancer networks.Nature cell biology · 2019Article
- mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells.Scientific reports · 2019Article
- Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development.Cell reports · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 5 institutions in 3 countries.
Funding
Abstract
Human embryonic stem cells (hESCs) display substantial heterogeneity in gene expression, implying the existence of discrete substates within the stem cell compartment. To determine whether these substates impact fate decisions of hESCs we used a GFP reporter line to investigate the properties of fractions of putative undifferentiated cells defined by their differential expression of the endoderm transcription factor, GATA6, together with the hESC surface marker, SSEA3. By single-cell cloning, we confirmed that substates characterized by expression of GATA6 and SSEA3 include pluripotent stem cells capable of long-term self-renewal. When clonal stem cell colonies were formed from GATA6-positive and GATA6-negative cells, more of those derived from GATA6-positive cells contained spontaneously differentiated endoderm cells than similar colonies derived from the GATA6-negative cells. We characterized these discrete cellular states using single-cell transcriptomic analysis, identifying a potential role for SOX17 in the establishment of the endoderm-biased stem cell state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.