ArticleTuberculosis (Edinburgh, Scotland)2018
Accuracy of whole genome sequencing versus phenotypic (MGIT) and commercial molecular tests for detection of drug-resistant Mycobacterium tuberculosis isolated from patients in Brazil and Mozambique.
Article in Tuberculosis (Edinburgh, Scotland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Xpert MTB/RIF assay for the diagnosis of rifampicin resistance in different regions: a meta-analysis.BMC microbiology · 2019Pooled it
- The Impact of Adopting Whole-Genome Sequencing for Tuberculosis Diagnosis in China: A Cost-Effectiveness Analysis.Open forum infectious diseases · 2026Article
- Beyond Hotspot Mutations: Diagnostic Relevance of High Frequency, Low Frequency, and DisputedPathogens (Basel, Switzerland) · 2025Review
- A Genome-Focused Investigation Reveals the Emergence of aMicroorganisms · 2024Article
- Monitoring of First-line Drug Resistance Mutations Outside the Scope of Xpert MTB/RIF Ultra is Needed for Successful Control of DR-TB in Southern Mozambique.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2024Article
- A comparison of phenotypic and WGS drug susceptibility testing inJAC-antimicrobial resistance · 2023Article
- Mycobacterium tuberculosis Lineages Associated with Mutations and Drug Resistance in Isolates from India.Microbiology spectrum · 2022Article
- Whole-Genome Sequencing Reveals Recent Transmission of Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2021Article
- Whole genome sequencing of clinical samples reveals extensively drug resistant tuberculosis (XDR TB) strains from the Beijing lineage in Nigeria, West Africa.Scientific reports · 2021Article
- Molecular epidemiology of Mycobacterium tuberculosis in Brazil before the whole genome sequencing era: a literature review.Memorias do Instituto Oswaldo Cruz · 2021Review
- Evaluation of drug susceptibility profile of Mycobacterium tuberculosis Lineage 1 from Brazil based on whole genome sequencing and phenotypic methods.Memorias do Instituto Oswaldo Cruz · 2021Article
- Diagnosis and treatment of multidrug-resistant tuberculosis.Yeungnam University journal of medicine · 2020Article
- Phylogenomic assessment of drug-resistant Mycobacterium tuberculosis strains from Beira, Mozambique.Tuberculosis (Edinburgh, Scotland) · 2020Article
- Whole genome sequencing of Mycobacterium tuberculosis isolates and clinical outcomes of patients treated for multidrug-resistant tuberculosis in Tanzania.BMC genomics · 2020Article
- QuantTB - a method to classify mixed Mycobacterium tuberculosis infections within whole genome sequencing data.BMC genomics · 2020Article
- Detection of Resistance to Fluoroquinolones and Second-Line Injectable Drugs AmongInfection and drug resistance · 2020Article
- Frequency of first and second-line drug resistance-associated mutations among resistant Mycobacterium tuberculosis clinical isolates from São Paulo, Brazil.Memorias do Instituto Oswaldo Cruz · 2020Article
- Xpert MTB/RIF performance to diagnose tuberculosis and rifampicin resistance in a reference centre in southern Brazil.ERJ open research · 2019Article
- Tuberculosis drug resistance profiling based on machine learning: A literature review.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesReview
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8 authors.
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Abstract
backgroundThe fast and accurate diagnosis of drug-resistant tuberculosis (DR-TB) is critical to reducing the spread of disease. Although commercial genotypic drug-susceptibility tests (DST) are close to the goal, they are still not able to detect all relevant DR-TB related mutations. Whole genome sequencing (WGS) allows better comprehension of DR-TB with a great discriminatory power. We aimed to evaluate WGS in M. tuberculosis isolates compared with phenotypic and genotypic DST.
methodsThis cross-sectional study evaluated 30 isolates from patients with detected DR-TB in Brazil and Mozambique. They were evaluated with phenotypic (MGIT-SIRE™) and genotypic (Xpert-MTB/RIF™, Genotype-MTBDRplus™, and MTBDRsl™) DST. Isolates with resistance to at least one first- or second-line drug were submitted to WGS and analyzed with TB profiler database.
resultsWGS had the best performance among the genotypic DST, compared to the phenotypic test. There was a very good concordance with phenotypic DST for rifampicin and streptomycin (89.6%), isoniazid (96.5%) and ethambutol (82.7%). WGS sensitivity and specificity for detection resistance were respectively 87.5 and 92.3% for rifampicin; 95.6 and 100% for isoniazid; 85.7 and 93.3% for streptomycin while 100 and 77.2% for ethambutol. Two isolates from Mozambique showed a Val170Phe rpoB mutation which was neither detected by Xpert-MTB/RIF nor Genotype-MTBDRplus.
conclusionWGS was able to provide all the relevant information about M. tuberculosis drug susceptibility in a single test and also detected a mutation in rpoB which is not covered by commercial genotypic DST.
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