Evidence map›Paper›PMID 29779067›Full record

ArticleJournal of biomolecular NMR2018

Cost-effective large-scale expression of proteins for NMR studies.

Julia Klopp, Aurélie Winterhalter, Rémy Gébleux, Daniela Scherer-Becker, Christian Ostermeier, Alvar D Gossert

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Journal of biomolecular NMR, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Julia KloppNovartis AG, 4002, Basel, Switzerland.
Aurélie WinterhalterNovartis AG, 4002, Basel, Switzerland.
Rémy GébleuxNovartis AG, 4002, Basel, Switzerland.
Daniela Scherer-BeckerNovartis AG, 4002, Basel, Switzerland.
Christian OstermeierNovartis AG, 4002, Basel, Switzerland.
Alvar D GossertInstitute for Molecular Biology and Biophysics, ETH Zürich, HPP L25.1, Hönggerbergring 24, 8093, Zurich, Switzerland. alvar.gossert@mol.biol.ethz.ch.
Novartis (Switzerland) · CHETH Zurich · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present protocols for high-level expression of isotope-labelled proteins in E. coli in cost-effective ways. This includes production of large amounts of unlabeled proteins and

Indexed as

Carbon IsotopesDeuteriumEscherichia coliFermentationIsotope LabelingMethionineNitrogen IsotopesNuclear Magnetic Resonance, BiomolecularReproducibility of ResultsCarbon IsotopesDeuteriumMethioninemethionine methyl esterNitrogen IsotopesAuto-inductionCost-effectiveHigh cell-density fermentationIsotope labelingMethionine labelling

Identifiers

PMID29779067
OpenAlexW2803297131

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.