Evidence map›Paper›PMID 29779063›Full record

ArticleFish physiology and biochemistry2018

Development of sperm vitrification protocols for two endangered salmonid species: the Adriatic grayling, Thymallus thymallus, and the marble trout, Salmo marmoratus.

Eszter Kása, Jelena Lujić, Zoran Marinović, Tímea Kollár, Gergely Bernáth, Zoltán Bokor, Béla Urbányi, Kinga Katalin Lefler, Dušan Jesenšek, Ákos Horváth

Abstract read
PubMed Publisher
In one paragraph

Article in Fish physiology and biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Eszter KásaDepartment of Aquaculture, Szent István University, Páter Károly u. 1., Gödöllő, 2100, Hungary. kasa.eszter@mkk.szie.hu.
Jelena LujićDepartment of Aquaculture, Szent István University, Páter Károly u. 1., Gödöllő, 2100, Hungary.
Zoran MarinovićDepartment of Aquaculture, Szent István University, Páter Károly u. 1., Gödöllő, 2100, Hungary.
Tímea KollárDepartment of Aquaculture, Szent István University, Páter Károly u. 1., Gödöllő, 2100, Hungary.
Gergely BernáthDepartment of Aquaculture, Szent István University, Páter Károly u. 1., Gödöllő, 2100, Hungary.
Zoltán BokorDepartment of Aquaculture, Szent István University, Páter Károly u. 1., Gödöllő, 2100, Hungary.
Béla UrbányiDepartment of Aquaculture, Szent István University, Páter Károly u. 1., Gödöllő, 2100, Hungary.
Kinga Katalin LeflerDepartment of Aquaculture, Szent István University, Páter Károly u. 1., Gödöllő, 2100, Hungary.
Dušan JesenšekRibiška družina Tolmin, Trg 1. maja 7, 5220, Tolmin, Slovenia.
Ákos HorváthDepartment of Aquaculture, Szent István University, Páter Károly u. 1., Gödöllő, 2100, Hungary.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vitrification was applied to the sperm of two endangered fish species of Soča River basin in Slovenia, the Adriatic grayling (Thymallus thymallus) and marble trout (Salmo marmoratus) following testing different cooling devices and vitrifying media. Sperm was collected, diluted in species-specific non-activating media containing cryoprotectants, and vitrified by plunging directly into liquid nitrogen without pre-cooling. Progressive motility, curvilinear velocity, and straightness of fresh and vitrified-warmed sperm were evaluated with computer-assisted sperm analysis (CASA). Fertilization trials were carried out to test the effectiveness of vitrification in the case of grayling. A protocol utilizing a glucose-based extender, 30% cryoprotectants (15% methanol + 15% propylene glycol), 1:1 dilution ratio, and droplets of 2 μl on a Cryotop as cooling device yielded the highest post-thaw motility values for both Adriatic grayling (7.5 ± 6.5%) and marble trout (26.6 ± 15.8%). Viable embryos were produced by fertilizing eggs with vitrified grayling sperm (hatching 13.1 ± 11.7%, control hatching 73.9 ± 10.4%). The vitrification protocol developed in this study can be utilized in the conservation efforts for the two species as an alternative to slow-rate freezing when working in field conditions or when specific equipment necessary for slow-rate freezing is not available.

Indexed as

Endangered SpeciesSperm MotilityVitrificationAnimalsCryopreservationCryoprotective AgentsFertilizationMaleSalmonidaeSemen AnalysisSemen PreservationCryoprotective AgentsAdriatic graylingFish sperm cryopreservationGene preservationMarble troutSpermatozoa vitrificationUltra-rapid cooling

Identifiers

PMID29779063

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.