Evidence map›Paper›PMID 29779031›Full record

ArticleJournal of vascular research2018

A Novel ex vivo Mouse Mesometrium Culture Model for Investigating Angiogenesis in Microvascular Networks.

Ariana D Suarez-Martinez, Susanne Bierschenk, Katie Huang, Dana Kaplan, Carolyn L Bayer, Stryder M Meadows, Markus Sperandio, Walter L Murfee

Open access · bronzeAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of vascular research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Pericyte migration and proliferation are tightly synchronized to endothelial cell sprouting dynamics.Integrative biology : quantitative biosciences from nano to macro · 2021
    Article
  7. Induction of microvascular network growth in the mouse mesentery.Microcirculation (New York, N.Y. : 1994) · 2018
    Article
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Ariana D Suarez-MartinezJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, USA.
Susanne BierschenkWalter-Brendel-Centre of Experimental Medicine, Klinikum der Universität München, Ludwig-Maximilians-Universität, Munich, Germany.
Katie HuangDepartment of Cell and Molecular Biology, Tulane University, New Orleans, Louisiana, USA.
Dana KaplanDepartment of Biomedical Engineering, Tulane University, New Orleans, Louisiana, USA.
Carolyn L BayerDepartment of Biomedical Engineering, Tulane University, New Orleans, Louisiana, USA.
Stryder M MeadowsDepartment of Cell and Molecular Biology, Tulane University, New Orleans, Louisiana, USA.
Markus SperandioWalter-Brendel-Centre of Experimental Medicine, Klinikum der Universität München, Ludwig-Maximilians-Universität, Munich, Germany.
Walter L MurfeeJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, USA.
Tulane University · USLudwig-Maximilians-Universität München · DE

Funding

Angiogenesis Model for Aging ResearchR01AG049821 · NIA · UNIVERSITY OF FLORIDA · PI MURFEE, WALTER L · 2016 to 2019
$1.0M
NIA NIH HHS R01 AG049821
6 · The paper itself

Abstract

backgroundThe development of models that incorporate intact microvascular networks enables the investigation of multicellular dynamics during angiogenesis. Our laboratory introduced the rat mesentery culture model as such a tool, which would be enhanced with mouse tissue. Since mouse mesentery is avascular, an alternative is mouse mesometrium, the connective tissue of uterine horns. The study's objective was to demonstrate that mouse mesometrium contains microvascular networks that can be cultured to investigate multicellular dynamics during angiogenesis.

methodsHarvested mesometrium tissues from C57Bl/6 female mice were cultured in media with serum for up to 7 days. PECAM, NG2, αSMA, and LYVE-1 labeling identified endothelial cells, pericytes, smooth muscle cells, and lymphatic endothelial cells, respectively.

resultsThese cells comprised microvascular networks with arterioles, venules, and capillaries. Compared to day 0, capillary sprouts per vascular length were increased by 3 and 5 days in culture (day 0, 0.08 ± 0.01; day 3, 3.19 ± 0.78; day 5, 2.49 ± 0.05 sprouts/mm; p < 0.05). Time-lapse imaging of cultured tissues from FlkEGFP mice showcases the use of the model for lineage studies. The impact is supported by the identification of endothelial cell jumping from one sprout to another.

conclusionThese results introduce a novel culture model for investigating multicellular dynamics during angiogenesis in real-time ex vivo microvascular networks.

Indexed as

Neovascularization, PhysiologicActinsAnimalsAntigensBiomarkersChondroitin Sulfate Proteoglycan 4FemaleGlycoproteinsGreen Fluorescent ProteinsMembrane Transport ProteinsMice, Inbred C57BLMice, TransgenicMicrovesselsModels, AnimalPlatelet Endothelial Cell Adhesion Molecule-1ProteoglycansActinsalpha-smooth muscle actin, mouseAntigensBiomarkersChondroitin Sulfate Proteoglycan 4enhanced green fluorescent proteinGlycoproteinsGreen Fluorescent ProteinsLYVE1 protein, mouseMembrane Transport ProteinsPlatelet Endothelial Cell Adhesion Molecule-1ProteoglycansVascular Endothelial Growth Factor Avascular endothelial growth factor A, mouseAngiogenesisCapillary sproutingEndothelial cellMicrocirculationMicrovascular networkPericyte

Identifiers

PMID29779031
PMCPMC6314041
OpenAlexW2805026728

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.