Evidence map›Paper›PMID 29778665›Full record

ArticleBiochimica et biophysica acta. Molecular and cell biology of lipids2018

A novel system to quantify intestinal lipid digestion and transport.

Øystein Sæle, Kari Elin L Rød, Vanessa H Quinlivan, Shengrong Li, Steven A Farber

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochimica et biophysica acta. Molecular and cell biology of lipids, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
3.1field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 26 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 2 countries.

Øystein SæleInstitute of Marine Research, Strandgaten 229, 5004 Bergen, Norway. Electronic address: oystein.saele@hi.no.
Kari Elin L RødInstitute of Marine Research, Strandgaten 229, 5004 Bergen, Norway.
Vanessa H QuinlivanDepartment of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA; The Johns Hopkins University, Department of Biology, Baltimore, MD 21218, USA.
Shengrong LiAvanti Polar Lipids, Inc., 700 Industrial Park Drive, Alabaster, AL 35007-9105, USA.
Steven A FarberDepartment of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA; The Johns Hopkins University, Department of Biology, Baltimore, MD 21218, USA. Electronic address: farber@ciwemb.edu.
Norwegian Institute of Marine Research · NOAvanti Polar Lipids (United States) · USCarnegie Institution for Science · USDepartment of Embryology · US

Funding

CELLULAR AND MOLECULAR BIOLOGYT32GM007231 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI VAN DOREN, MARK B · 1985 to 2020
$18.4M
Systematic Vertebrate Functional GenomicsR01GM063904 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI EKKER, STEPHEN CARL · 2001 to 2025
$10.7M
Identifying genes required for digestive physiology and lipid metabolismR01DK093399 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI STEVEN A FARBER, John F Rawls · 2013 to 2026
$7.6M
Cellular and Molecular BiologyT32GM141804 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI John Kim · 2021 to 2026
$5.1M
Acquisition of GE Typhoon FLA9500 Variable Mode ImagerS10OD021567 · OD · JOHNS HOPKINS UNIVERSITY · PI MCCAFFERY, J. MICHAEL · 2017 to 2017
$147k
In vivo imaging to evaluate the effect of PUFAs on alcoholic fatty liver diseaseF31AA023142 · NIAAA · JOHNS HOPKINS UNIVERSITY · PI QUINLIVAN-REPASI, VANESSA HELEN · 2014 to 2016
$129k
NIAAA NIH HHS F31 AA023142NIDDK NIH HHS R01 DK093399NIGMS NIH HHS R01 GM063904NIGMS NIH HHS T32 GM007231NIGMS NIH HHS T32 GM141804NIH HHS S10 OD021567
6 · The paper itself

Abstract

The zebrafish larva is a powerful tool for the study of dietary triglyceride (TG) digestion and how fatty acids (FA) derived from dietary lipids are absorbed, metabolized and distributed to the body. While fluorescent FA analogues have enabled visualization of FA metabolism, methods for specifically assaying TG digestion are badly needed. Here we present a novel High Performance Liquid Chromatography (HPLC) method that quantitatively differentiates TG and phospholipid (PL) molecules with one or two fluorescent FA analogues. We show how this tool may be used to discriminate between undigested and digested TG or phosphatidylcholine (PC), and also the products of TG or PC that have been digested, absorbed and re-synthesized into new lipid molecules. Using this approach, we explored the dietary requirement of zebrafish larvae for phospholipids. Here we demonstrate that dietary TG is digested and absorbed in the intestinal epithelium, but without dietary PC, TG accumulates and is not transported out of the enterocytes. Consequently, intestinal ER stress increases and the ingested lipid is not available support the energy and metabolic needs of other tissues. In TG diets with PC, TG is readily transported from the intestine and subsequently metabolized.

Indexed as

AnimalsBoron CompoundsChromatography, High Pressure LiquidDietary FatsEnterocytesFatty AcidsFluorescent DyesIntestinal AbsorptionIntestinal MucosaIntestinesLarvaLipid DropletsLipid MetabolismPhosphatidylcholinesTriglyceridesZebrafish4,4-difluoro-4-bora-3a,4a-diaza-s-indaceneBoron CompoundsDietary FatsFatty AcidsFluorescent DyesPhosphatidylcholinesTriglyceridesLipase/digestiveLipid dropletsNutritionPhospholipids/absorptionTriglyceridesZebrafish

Identifiers

PMID29778665
PMCPMC6054555
OpenAlexW2803985767

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.