Evidence map›Paper›PMID 29777751›Full record

ArticleMethods (San Diego, Calif.)2019

Evaluating the susceptibility of AGO2-loaded microRNAs to degradation by nucleases in vitro.

Reyad A Elbarbary, Lynne E Maquat

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods (San Diego, Calif.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 59% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Reyad A ElbarbaryDepartment of Orthopaedics and Rehabilitation, Penn State University, College of Medicine, Hershey, PA 17033-0850, USA; Department of Biochemistry and Molecular Biology, Penn State University, College of Medicine, Hershey, PA 17033-0850, USA. Electronic address: relbarbary@pennstatehealth.psu.edu.
Lynne E MaquatDepartment of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA; Center for RNA Biology, University of Rochester, Rochester, NY 14642, USA. Electronic address: lynne_maquat@urmc.rochester.edu.
Penn State Milton S. Hershey Medical Center · USUniversity of Rochester · US

Funding

Regulation of Normal and Defective Human GenesR37GM074593 · NIGMS · UNIVERSITY OF ROCHESTER · PI MAQUAT, LYNNE E · 2014 to 2023
$4.8M
Regulation of Normal and Defective Human GenesR01GM074593 · NIGMS · UNIVERSITY OF ROCHESTER · PI MAQUAT, LYNNE E · 2006 to 2013
$3.1M
Acquisition of a Typhoon FLA 9500 Laser Scanning ImagerS10OD021489 · OD · UNIVERSITY OF ROCHESTER · PI BUTLER, JAMES SCOTT · 2017 to 2017
$130k
NIGMS NIH HHS R01 GM074593NIGMS NIH HHS R37 GM074593NIH HHS S10 OD021489
6 · The paper itself

Abstract

MicroRNAs (miRNAs) comprise a class of small non-coding RNAs that regulate the stability and/or translatability of most protein-coding transcripts. Steady-state levels of mature miRNAs can be controlled through mechanisms that influence their biogenesis and/or decay rates. Pathways that mediate mature miRNA decay are less well understood than those that mediate miRNA biogenesis. We recently described Tudor-staphylococcal/micrococcal-like nuclease (TSN)-mediated miRNA decay (TumiD) as a cellular pathway that promotes the sequence-specific endonucleolytic decay of miRNAs that harbor a CA and/or UA dinucleotide. Here, we describe an in vitro assay for evaluating the susceptibility of AGO2-loaded miRNAs to degradation by different classes of nucleases. This in vitro approach can be used to complement in vivo studies that aim to identify novel miRNA decay factors.

Indexed as

RNA StabilityArgonaute ProteinsBiochemistryEndonucleasesHEK293 CellsHumansMicroRNAsNuclear ProteinsRibonuclease, PancreaticAGO2 protein, humanArgonaute ProteinsEndonucleasesMicroRNAsNuclear ProteinsRibonuclease, PancreaticSND1 protein, humanAGO2ImmunoprecipitationIn vitro assaymiRNA decayNucleaseTudor-SN

Identifiers

PMID29777751
PMCPMC6240400
OpenAlexW2803626990

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.