Evidence map›Paper›PMID 29777440›Full record

ArticleDigestive diseases and sciences2018

MiR-27a/b Regulates Liver Regeneration by Posttranscriptional Modification of Tmub1.

Xiang Lan, Guangyao Li, Hongming Liu, Hangwei Fu, Ping Chen, Menggang Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Digestive diseases and sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. The Functional Mechanism of MicroRNA in Oral Lichen Planus.Journal of inflammation research · 2022
    Review
  3. [Research progress on the relationship between non-coding RNA and liver regeneration].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2021
    Review
  4. Review
  5. Cell Sources and Influencing Factors of Liver Regeneration: A Review.Medical science monitor : international medical journal of experimental and clinical research · 2020
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Xiang LanDepartment of Hepatobiliary Surgery, Daping Hospital, The Third Military Medical University (Army Medical University), 10# Changjiangzhilu Daping, Chongqing, 400042, China.
Guangyao LiDepartment of Hepatobiliary Surgery, Daping Hospital, The Third Military Medical University (Army Medical University), 10# Changjiangzhilu Daping, Chongqing, 400042, China.
Hongming LiuDepartment of Hepatobiliary Surgery, Daping Hospital, The Third Military Medical University (Army Medical University), 10# Changjiangzhilu Daping, Chongqing, 400042, China.
Hangwei FuDepartment of Hepatobiliary Surgery, Daping Hospital, The Third Military Medical University (Army Medical University), 10# Changjiangzhilu Daping, Chongqing, 400042, China.
Ping ChenDepartment of Hepatobiliary Surgery, Daping Hospital, The Third Military Medical University (Army Medical University), 10# Changjiangzhilu Daping, Chongqing, 400042, China.
Menggang LiuDepartment of Hepatobiliary Surgery, Daping Hospital, The Third Military Medical University (Army Medical University), 10# Changjiangzhilu Daping, Chongqing, 400042, China. Menggang_liu@hotmail.com.
Army Medical University · CNDaping Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTransmembrane and ubiquitin-like domain-containing 1 protein (Tmub1) negatively regulates liver regeneration. However, whether this regulation involves posttranscriptional modification of Tmub1 expression is unknown.

aimThe aim of the study was to investigate whether microRNA (miR)-27a/b regulates posttranscriptional modification of Tmub1 and cell proliferation during liver regeneration.

methodsTmub1 mRNA 3'-untranslated region (UTR) sequences were analyzed using online software. A luciferase assay was used to verify the relationship between miR-27a/b and the 3'-UTR of Tmub1. Rat partial hepatectomy models were used to investigate miR-27a/b and Tmub1 levels after partial hepatectomy. MiR-27a/b expression was down- and up-regulated with mimics and inhibitors, respectively, to observe the effects of miR-27a/b on Tmub1 expression. Quantitative RT-PCR and Western blot analyses were used to measure miR-27a/b and Tmub1 expression. Hepatocyte proliferation was measured using the CCK8 method for BRL-3A liver cells and proliferating cell nuclear antigen and histone H3 phosphorylation in the regenerating liver.

resultsA potential binding site of miR-27a/b was found in the 3'-UTR sequence of Tmub1. Our luciferase assay confirmed that the Tmub1 mRNA 3'-UTR was the target of miR-27a/b. We observed a temporal correlation between miR-27a/b and Tmub1 expression during liver regeneration. MiR-27a/b down-regulated Tmub1 expression both in vivo and in vitro. MiR-27a/b regulated hepatocyte proliferation during liver regeneration.

conclusionMiR-27a/b regulates hepatocyte proliferation by controlling posttranscriptional modification of Tmub1 during liver regeneration.

Indexed as

Cell ProliferationLiver RegenerationRNA Processing, Post-Transcriptional3' Untranslated RegionsAnimalsBinding SitesCarrier ProteinsCell LineHepatectomyHepatocytesHistonesLiverMicroRNAsModels, AnimalNuclear ProteinsPhosphorylation3' Untranslated RegionsCarrier ProteinsHistonesMicroRNAsMIRN27 microRNA, ratNuclear ProteinsProliferating Cell Nuclear AntigenTmub1 protein, ratLiver regenerationMiR-27a/bPosttranscriptional regulationTmub1

Identifiers

PMID29777440
OpenAlexW2804913737

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.