Evidence map›Paper›PMID 29777416›Full record

ArticleFish physiology and biochemistry2018

Identification of genes involved in gonadal sex differentiation and the dimorphic expression pattern in Takifugu rubripes gonad at the early stage of sex differentiation.

Hongwei Yan, Xufang Shen, Xin Cui, Yumeng Wu, Lianshun Wang, Lei Zhang, Qi Liu, Yusheng Jiang

Abstract read
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In one paragraph

Article in Fish physiology and biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
8.0field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 52 citations in OpenAlex.

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  14. Evolution, Expression, and Function of Gonadal Somatic Cell-Derived Factor.Frontiers in cell and developmental biology · 2021
    Review
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  20. A T > G Mutation in theFrontiers in genetics · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Hongwei YanCollege of Fisheries and life Science, Dalian Ocean University, No. 52 Heishijiao Street, Shahekou District, Dalian, 116023, China.
Xufang ShenCollege of Fisheries and life Science, Dalian Ocean University, No. 52 Heishijiao Street, Shahekou District, Dalian, 116023, China.
Xin CuiCollege of Fisheries and life Science, Dalian Ocean University, No. 52 Heishijiao Street, Shahekou District, Dalian, 116023, China.
Yumeng WuCollege of Fisheries and life Science, Dalian Ocean University, No. 52 Heishijiao Street, Shahekou District, Dalian, 116023, China.
Lianshun WangCollege of Fisheries and life Science, Dalian Ocean University, No. 52 Heishijiao Street, Shahekou District, Dalian, 116023, China.
Lei ZhangCollege of Marine Science and Environment Engineering, Dalian Ocean University, No. 52 Heishijiao Street, Shahekou District, Dalian, 116023, China.
Qi LiuCollege of Marine Science and Environment Engineering, Dalian Ocean University, No. 52 Heishijiao Street, Shahekou District, Dalian, 116023, China. liuqisunson@163.com.ORCID http://orcid.org/0000-0002-4730-4736
Yusheng JiangCollege of Fisheries and life Science, Dalian Ocean University, No. 52 Heishijiao Street, Shahekou District, Dalian, 116023, China.
Dalian Ocean University · CN

Funding

National Key Research and Development Project 2017YFB0404000
6 · The paper itself

Abstract

Quantifying the expression of mRNAs in the gonads at the critical stage of molecular sex differentiation stage might help to clarify the regulatory network during early sex differentiation and provide new information on the role of sex-related genes in gonadal function. In this study, transcriptomic analysis of sex-related genes expression profiles in fugu gonads at 60 and 90 days after hatching (dah) was conducted firstly, and a total of 112,504,991 clean reads, encompassing 28.35 Gb of sequences were retrieved. Twenty-three thousand eight hundred ten genes were found to be expressed in juvenile fugu gonads, and we mainly focused on the differentially expressed genes that have the potential to be involved in the gonadal sex differentiation. For 60-dah juveniles, we identified 1014 genes that were upregulated in the ovary and 1570 that were upregulated in the testis. For 90-dah juveniles, we identified 1287 genes that were upregulated in the ovary and 1500 that were upregulated in the testis. The dimorphic expression patterns of 15 genes in gonads at 30 and 40 dah were further investigate using qPCR. Cyp11b and star were expressed at higher levels in XY than in XX, while cyp11a1 and cyp19a1a were expressed at higher levels in XX than in XY at 30 dah. At 40 dah, the levels of gsdf, dmrt1, dmrt3, cyp11c1, star, and hsd3b expression were higher in XY, while the levels of foxl2, cyp19a1a, wnt9b, and foxD4 expression were higher in XX. Sox9, cyp11a1, cyp17a1, cyp17a2, and nr5a2 were expressed at similar levels in XX and XY at 40 dah. This is the first report of gonadal transcriptome of fugu at early sex differentiation stage, and our results provide an archive for further study on molecular mechanism underlying sex differentiation in this species.

Indexed as

AgingAnimalsFemaleGene Expression Regulation, DevelopmentalGonadsMaleRNASex DifferentiationSexual MaturationTakifuguTranscriptomeRNADifferentially expressed genesEarly sex differentiationGonadal transcriptome analysisTakifugu rubripes

Identifiers

PMID29777416
OpenAlexW2803762014

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.