ArticleApplied and environmental microbiology2018
Enrichment of Clinically Relevant Organisms in Spontaneous Preterm-Delivered Placentas and Reagent Contamination across All Clinical Groups in a Large Pregnancy Cohort in the United Kingdom.
Article in Applied and environmental microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed, 79 citations in OpenAlex.
- The Gut Microbiome in Early Ontogeny: Implications for Brain and Immune System Development.Journal of developmental biology · 2026Review
- Critical assessment of intratumor and low-biomass microbiome using long-read sequencing.bioRxiv : the preprint server for biology · 2026Article
- Early-life gut microbiome development and its potential long-term impact on health outcomes.Microbiome research reports · 2025Review
- Validating digital polymerase chain reaction for 16S rRNA gene amplification from low biomass environmental samples.ISME communications · 2025Article
- Evaluation of the Human Placental Microbiota in Early- and Late-Onset Pre-Eclampsia.High blood pressure & cardiovascular prevention : the official journal of the Italian Society of Hypertension · 2024Article
- Neurodevelopmental Disorders Associated with Gut Microbiome Dysbiosis in Children.Children (Basel, Switzerland) · 2024Review
- Urinary microbiome community types associated with urinary incontinence severity in women.American journal of obstetrics and gynecology · 2024Article
- Analysis of the microbiome in maternal, intrauterine and fetal environments based on 16S rRNA genes following different durations of membrane rupture.Scientific reports · 2023Article
- In utero priming of fetal immune activation: Myths and mechanisms.Journal of reproductive immunology · 2023Review
- Review
- Is there a placental microbiota? A critical review and re-analysis of published placental microbiota datasets.BMC microbiology · 2023Review
- Assessment of the human placental microbiome in early pregnancy.Frontiers in medicine · 2023Article
- Next Generation Sequencing Approaches to Characterize the Respiratory Tract Virome.Microorganisms · 2022Review
- Crucial nuances in understanding (mis)associations between the neonatal microbiome and Cesarean delivery.Trends in molecular medicine · 2022Review
- Maternal septicemia caused by Streptococcus mitis: a possible link between intra-amniotic infection and periodontitis. Case report and literature review.BMC infectious diseases · 2022Review
- Maternal and early life exposures and their potential to influence development of the microbiome.Genome medicine · 2022Review
- Virome and metagenomic analysis reveal the distinct distribution of microbiota in human fetal gut during gestation.Frontiers in immunology · 2022Article
- First encounters of the microbial kind: perinatal factors direct infant gut microbiome establishment.Microbiome research reports · 2022Review
- Oral microbiome shifts during pregnancy and adverse pregnancy outcomes: Hormonal and Immunologic changes at play.Periodontology 2000 · 2021Review
- The reproductive tract microbiota in pregnancy.Bioscience reports · 2021Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
In this study, differences in the placental microbiota from term and preterm deliveries in a large pregnancy cohort in the United Kingdom were studied by using 16S-targeted amplicon sequencing. The impacts of contamination from DNA extraction, PCR reagents, and the delivery itself were also examined. A total of 400 placental samples from 256 singleton pregnancies were analyzed, and differences between spontaneous preterm-, nonspontaneous preterm-, and term-delivered placentas were investigated. DNA from recently delivered placentas was extracted, and screening for bacterial DNA was carried out by using targeted sequencing of the 16S rRNA gene on the Illumina MiSeq platform. Sequenced reads were analyzed for the presence of contaminating operational taxonomic units (OTUs) identified via sequencing of negative extraction and PCR-blank samples. Differential abundances and between-sample (beta) diversity metrics were then compared. A large proportion of the reads sequenced from the extracted placental samples mapped to OTUs that were also found for negative extractions. Striking differences in the compositions of samples were also observed, according to whether the placenta was delivered abdominally or vaginally, providing strong circumstantial evidence for delivery contamination as an important contributor to observed microbial profiles. When OTU- and genus-level abundances were compared between the groups of interest, a number of organisms were enriched in the spontaneous preterm-delivery cohort, including organisms that have been associated previously with adverse pregnancy outcomes, specifically
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.