ArticleMicrobiome2018
Fecal Aliquot Straw Technique (FAST) allows for easy and reproducible subsampling: assessing interpersonal variation in trimethylamine-N-oxide (TMAO) accumulation.
Article in Microbiome, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Short-term high fat diet-induced metabolic endotoxemia in older individuals with obesity: a randomized crossover study.The American journal of clinical nutrition · 2025Trial
- Characterization of TMAO productivity from carnitine challenge facilitates personalized nutrition and microbiome signatures discovery.Microbiome · 2020Trial
- Microbial Transplantation With Human Gut Commensals Containing CutC Is Sufficient to Transmit Enhanced Platelet Reactivity and Thrombosis Potential.Circulation research · 2018Trial
- Phenotypic high-throughput screening identifies modulators of gut microbial choline metabolism.mBio · 2026Article
- Linking interpersonal differences in gut microbiota composition and drug biotransformation activity.bioRxiv : the preprint server for biology · 2026Article
- Supplementation with abioRxiv : the preprint server for biology · 2025Article
- Fecal short-chain fatty acids vary by sex and amyloid status.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2025Article
- Association between Exposure to Metals during Pregnancy, Childhood Gut Microbiome, and Risk of Intestinal Inflammation in Late Childhood.Environment & health (Washington, D.C.) · 2024Article
- Prenatal metal exposures and childhood gut microbial signatures are associated with depression score in late childhood.The Science of the total environment · 2024Article
- Prenatal Lead Exposure Is Associated with Reduced Abundance of Beneficial Gut Microbial Cliques in Late Childhood: An Investigation Using Microbial Co-Occurrence Analysis (MiCA).Environmental science & technology · 2023Article
- Article
- Prenatal Pb exposure is associated with reduced abundance of beneficial gut microbial cliques in late childhood: an investigation using Microbial Co-occurrence Analysis (MiCA).medRxiv : the preprint server for health sciences · 2023Article
- Prenatal Lead Exposure is Negatively Associated with the Gut Microbiome in Childhood.medRxiv : the preprint server for health sciences · 2023Article
- Isolation and Cultivation of Human Gut Microorganisms: A Review.Microorganisms · 2023Review
- Prenatal lead exposure is negatively associated with the gut microbiome in childhood.Frontiers in microbiology · 2023Article
- A gut microbial metabolite of dietary polyphenols reverses obesity-driven hepatic steatosis.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Gut microbiome variation modulates the effects of dietary fiber on host metabolism.Microbiome · 2021Article
- Standardization of microbiome studies for urolithiasis: an international consensus agreement.Nature reviews. Urology · 2021Article
- Gut-derived Flavonifractor species variants are differentially enriched during in vitro incubation with quercetin.PloS one · 2020Article
- The Enhanced Pharmacological Effects of Modified Traditional Chinese Medicine in Attenuation of Atherosclerosis Is Driven by Modulation of Gut Microbiota.Frontiers in pharmacology · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundConvenient, reproducible, and rapid preservation of unique biological specimens is pivotal to their use in microbiome analyses. As an increasing number of human studies incorporate the gut microbiome in their design, there is a high demand for streamlined sample collection and storage methods that are amenable to different settings and experimental needs. While several commercial kits address collection/shipping needs for sequence-based studies, these methods do not preserve samples properly for studies that require viable microbes.
resultsWe describe the Fecal Aliquot Straw Technique (FAST) of fecal sample processing for storage and subsampling. This method uses a straw to collect fecal material from samples recently voided or preserved at low temperature but not frozen (i.e., 4 °C). Different straw aliquots collected from the same sample yielded highly reproducible communities as disclosed by 16S rRNA gene sequencing; operational taxonomic units that were lost, or gained, between the two aliquots represented very low-abundance taxa (i.e., < 0.3% of the community). FAST-processed samples inoculated into germ-free animals resulted in gut communities that retained on average ~ 80% of the donor's bacterial community. Assessment of choline metabolism and trimethylamine-N-oxide accumulation in transplanted mice suggests large interpersonal variation.
conclusionsOverall, FAST allows for repetitive subsampling without thawing of the specimens and requires minimal supplies and storage space, making it convenient to utilize both in the lab and in the field. FAST has the potential to advance microbiome research through easy, reproducible sample processing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.