ArticleMicrobial cell factories2018
Identification, function, and application of 3-ketosteroid Δ1-dehydrogenase isozymes in Mycobacterium neoaurum DSM 1381 for the production of steroidic synthons.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 55 citations in OpenAlex.
- Stereoselective 15α-hydroxylation of androsta-1,4-diene-3,17-dione byRSC advances · 2026Article
- Enhancing the bioconversion of phytosterols to 22-hydroxy-23,24- bisnorchol-4-ene-3-one in Mycobacterium neoaurum ZS-15 through genetic modification of kstD1 and wecA.Microbial cell factories · 2026Article
- Rerouting phytosterol degradation pathway for directed androst-1,4-diene-3,17-dione microbial bioconversion.Applied microbiology and biotechnology · 2024Article
- Reconstruction of the Steroid 1(2)-Dehydrogenation System fromMicroorganisms · 2023Article
- Unravelling and engineering an operon involved in the side-chain degradation of sterols in Mycolicibacterium neoaurum for the production of steroid synthons.Biotechnology for biofuels and bioproducts · 2023Article
- Whole-Genome Analysis ofInternational journal of molecular sciences · 2023Article
- Improving the production of 9α-hydroxy-4-androstene-3,17-dione from phytosterols by 3-ketosteroid-ΔMicrobial cell factories · 2023Article
- New Insights into the Modification of the Non-Core Metabolic Pathway of Steroids inInternational journal of molecular sciences · 2023Review
- Loop pathways are responsible for tuning the accumulation of C19- and C22-sterol intermediates in the mycobacterial phytosterol degradation pathway.Microbial cell factories · 2023Article
- 1,2-Hydrogenation and Transhydrogenation Catalyzed by 3-Ketosteroid ΔInternational journal of molecular sciences · 2022Article
- Rational development of mycobacteria cell factory for advancing the steroid biomanufacturing.World journal of microbiology & biotechnology · 2022Review
- Steroid Metabolism in Thermophilic ActinobacteriumMicroorganisms · 2021Article
- Production of 11α-hydroxysteroids from sterols in a single fermentation step by Mycolicibacterium smegmatis.Microbial biotechnology · 2021Article
- Efficient conversion of phytosterols into 4-androstene-3,17-dione and its C1,2-dehydrogenized and 9α-hydroxylated derivatives by engineered Mycobacteria.Microbial cell factories · 2021Article
- Universal capability of 3-ketosteroid ΔMicrobial cell factories · 2021Article
- Biosynthesis and Industrial Production of Androsteroids.Plants (Basel, Switzerland) · 2020Review
- Enhancing the bioconversion of phytosterols to steroidal intermediates by the deficiency of kasB in the cell wall synthesis of Mycobacterium neoaurum.Microbial cell factories · 2020Article
- Improving phytosterol biotransformation at low nitrogen levels by enhancing the methylcitrate cycle with transcriptional regulators PrpR and GlnR of Mycobacterium neoaurum.Microbial cell factories · 2020Article
- Genome-wide response on phytosterol in 9-hydroxyandrostenedione-producing strain of Mycobacterium sp. VKM Ac-1817D.BMC biotechnology · 2019Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
background3-Ketosteroid-Δ1-dehydrogenase (KstD) is a key enzyme in the metabolic pathway for chemical modifications of steroid hormones. Only a few KstDs have thus far been characterized biochemically and applied for the production of steroidal pharmaceutical intermediates. Three KstDs, KstD1, KstD2, and KstD3, were identified in Mycobacterium neoaurum DSM 1381, and they shared up to 99, 85 and 97% amino acid identity with previously reported KstDs, respectively. In this paper, KstDs from M. neoaurum DSM 1381 were investigated and exemplified their potential application for industrial steroid transformation.
resultsThe recombinant KstD2 from Bacillus subtilis exhibited higher enzymatic activity when 4-androstene-3,17-dione (AD) and 22-hydroxy-23, 24-bisnorchol-4-ene-3-one (4HP) were used as the substrates, and resulted in specific activities of 22.40 and 19.19 U mg
conclusionsIn vitro assay employing the recombinant enzymes revealed that KstD2 was the most promising candidate for biocatalysis in biotransformation of AD. However, in vivo analysis showed that the cellular regulation of kstD1 was much more active than those of the other kstDs in response to the presence of phytosterols. Based on the findings above, we successfully constructed E. coli transformant BL21-kstD2 for ADD production from AD and M. neoaurum DSM 1381 ΔkstD1 strain for 4HP production using phytosterols as the substrate.
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