ArticleAnnals of clinical microbiology and antimicrobials2018
Rapid detection of carbapenemase activity of Enterobacteriaceae isolated from positive blood cultures by MALDI-TOF MS.
Article in Annals of clinical microbiology and antimicrobials, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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16 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Emerging strategies for the noninvasive diagnosis of nosocomial pneumonia.Expert review of anti-infective therapy · 2019Pooled it
- Carbapenem-resistant Klebsiella pneumoniae gut colonization and subsequent infection in pediatric intensive care units in shanghai, China.Annals of clinical microbiology and antimicrobials · 2025Article
- Mechanisms of Antimicrobial Resistance inInfection and drug resistance · 2025Review
- Development of a Simple Method to Detect the Carbapenemase-Producing GenesDiagnostics (Basel, Switzerland) · 2024Article
- Rapid Identification of Carbapenemase-ProducingInfection and drug resistance · 2023Article
- Clinical Performance of the Osmotic Shock-MALDI MS Method to Detect Klebsiella pneumoniae Carbapenemase in Clinical Isolates.Journal of clinical microbiology · 2022Article
- Multi Evaluation of a Modified GoldNano Carb Test for Carbapenemase Detection in Clinical Isolates of Gram-Negative Bacilli.Antibiotics (Basel, Switzerland) · 2022Article
- A rapid MALDI-TOF mass spectrometry-based method for colistin susceptibility testing in Escherichia coli.Microbial biotechnology · 2022Article
- Carbapenemase ProducingAntibiotics (Basel, Switzerland) · 2021Article
- Development of Loop-Mediated Isothermal Amplification Rapid Diagnostic Assays for the Detection ofFrontiers in molecular biosciences · 2021Article
- Rapid Detection ofFrontiers in cellular and infection microbiology · 2021Article
- Design of Rapid Detection System for Five Major Carbapenemase Families (Infection and drug resistance · 2021Article
- AI-Blue-Carba: A Rapid and Improved Carbapenemase Producer Detection Assay Using Blue-Carba With Deep Learning.Frontiers in microbiology · 2020Article
- Detection of carbapenemase producers by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS).European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Review
- Article
- Carbapenemases in Enterobacteriaceae: Detection and Antimicrobial Therapy.Frontiers in microbiology · 2019Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMatrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proved to be a useful tool for identification of pathogens directly isolated from blood cultures in clinical microbiology laboratories. β-Lactam antibiotics are commonly used for treatment of bloodstream infections caused by Enterobacteriaceae strains, and carbapenem is the superlative class of β-lactam antibiotics. Since the carbapenem resistance rate of Enterobacteriaceae strains raised year by year, efficient detection of carbapenemase activity and timely delivery of carbapenem susceptibility reports of Enterobacteriaceae strains isolated from blood cultures is important for clinicians.
methodsWe used 64 simulated blood cultures to establish the method of MALDI-TOF MS based ertapenem hydrolysis assay. The cutoff value of logRQ calculated from the peaks intensity of ertapenem and its hydrolysate was first set to identify the strains with carbapenemase activity. Then, we detected and calculated the logRQ values of 385 Enterobacteriaceae strains from positive clinical blood cultures to distinguish the carbapenemase producers and noncarbapenemase producers.
resultsThe mean logRQ value of 32 noncarbapenemase producers was - 0.85 ± 0.14 in simulated blood cultures, while the logRQ value of 32 carbapenemase producers was 0.87 ± 0.55. Thus, the cutoff value of logRQ was set at - 0.45 with sensitivity of 100% and specificity of 100%. In 385 clinical positive blood cultures, the logRQ values of all carbapenem-susceptible Enterobacteriaceae strains (81.3%, 313/385) were < - 0.45. Comparing with the detection of carbapenemase genes, carbapenem-resistant Enterobacteriaceae strains (18.7%, 72/385) were well distinguished by MALDI-TOF MS based ertapenem hydrolysis assay with a sensitivity of 92.5% and specificity of 100%.
conclusionsOur data show that MALDI-TOF MS based ertapenem hydrolysis assay is a rapid and accurate method to detect carbapenemase activity of Enterobacteriaceae strains from positive blood cultures, and can be routinely performed in clinical microbiology laboratories.
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