ArticleTranslational vision science & technology2018
Liquid Biopsy of Vitreous Reveals an Abundant Vesicle Population Consistent With the Size and Morphology of Exosomes.
Article in Translational vision science & technology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.
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Who cites it
44 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.
- Systematic review on biomarker potential of vitreous microRNA in retinal disease.Frontiers in ophthalmology · 2025Pooled it
- Exosomal miRNA Profiling in Liquid Biopsy of Vitreous in Proliferative Diabetic Retinopathy.Investigative ophthalmology & visual science · 2025Article
- Fibrotic Changes in Rhegmatogenous Retinal Detachment.International journal of molecular sciences · 2025Review
- Diagnostic and Therapeutic Utility of Extracellular Vesicles in Ocular Disease.International journal of molecular sciences · 2025Review
- Isolation and Characterization of Exosomes from Ocular Samples.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Extracellular vesicles as emerging players in glaucoma: Mechanisms, biomarkers, and therapeutic targets.Vision research · 2025Review
- A comprehensive review on recent advances in exosome isolation and characterization: Toward clinical applications.Translational oncology · 2024Review
- Enrichment protocols for human conjunctival extracellular vesicles and their characterization.Scientific reports · 2024Article
- Protein profile of purified plasma- and aqueous humor-derived extracellular vesicles from patients with ocular toxoplasmosis.Arquivos brasileiros de oftalmologia · 2024Article
- Assessment interocular symmetry of peripapillary vessel density in young myopes with optical coherence tomographic angiography.International ophthalmology · 2023Observational
- Roles of Optineurin and Extracellular Vesicles in Glaucomatous Retinal Cell Loss.Current medical science · 2023Article
- Exosomal MicroRNA Profiling in Vitreous Humor Derived From Pathological Myopia Patients.Investigative ophthalmology & visual science · 2023Article
- Recent advances of exosomes in age-related macular degeneration.Frontiers in pharmacology · 2023Review
- Mass Spectrometry-Based Vitreous Proteomics: Validated Methods and Analysis Pipeline.Methods in molecular biology (Clifton, N.J.) · 2023Article
- On the other end of the line: Extracellular vesicle-mediated communication in glaucoma.Frontiers in neuroanatomy · 2023Review
- Article
- Activity and Expression of Carboxylesterases and Arylacetamide Deacetylase in Human Ocular Tissues.Drug metabolism and disposition: the biological fate of chemicals · 2022Article
- Release of VAMP5-positive extracellular vesicles by retinal Müller glia in vivo.Journal of extracellular vesicles · 2022Article
- Proteomic Insight into the Role of Exosomes in Proliferative Vitreoretinopathy Development.Journal of clinical medicine · 2022Article
- Non-Exosomal and Exosome-Derived miRNAs as Promising Biomarkers in Canine Mammary Cancer.Life (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate the molecular components of the vitreous in order to better understand retinal physiology and disease.
methodsVitreous was acquired from patients undergoing vitrectomy for macular hole and/or epiretinal membrane, postmortem donors, and C57BL/6J mice. Unbiased proteomic analysis was performed via electrospray ionization tandem mass spectrometry (MS/MS). Gene ontology analysis was performed and results were confirmed with transmission electron microscopy, atomic force microscopy, and nanoparticle tracking analysis (NTA).
resultsProteomic analysis of vitreous obtained prior to vitrectomy identified a total of 1121 unique proteins. Gene ontology analysis revealed that 62.6% of the vitreous proteins were associated with the gene ontology term "extracellular exosome." Ultrastructural analyses, Western blot, and NTA confirmed the presence of an abundant population of vesicles consistent with the size and morphology of exosomes in human vitreous. The concentrations of vitreous vesicles in vitrectomy patients, postmortem donors, and mice were 1.3, 35, and 9 billion/mL, respectively.
conclusionsOverall, these data strongly suggest that information-rich exosomes are a major constituent of the vitreous. The abundance of these vesicles and the presence of retinal proteins imply a dynamic interaction between the vitreous and retina. Future studies will be required to identify the cellular origin of vitreal exosomes as well as to assess the potential role of these vesicles in retinal disease and treatment. TRANSLATIONAL RELEVANCE: The identification of vitreous exosomes lays the groundwork for a transformed understanding of pathophysiology and treatment mechanisms in retinal disease, and further validates the use of vitreous as a proximal biofluid of the retina.
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