ArticleScientific reports2018
Embryonic POU5F1 is Required for Expanded Bovine Blastocyst Formation.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed, 3 syntheses or guidelines pooled it, 115 citations in OpenAlex.
- Cross-species meta-analysis of transcriptome changes during the morula-to-blastocyst transition: metabolic and physiological changes take center stage.American journal of physiology. Cell physiology · 2021Pooled it
- Pooled it
- Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates.Frontiers in veterinary science · 2021Pooled it
- Molecular Regulation of Post-Hatching Embryo Development: Implications for Early Pregnancy Losses in Livestock Species.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Article
- Developmental origins of pregnancy loss in cattle.Animal reproduction · 2026Article
- Environmental and external stressors of postejaculatory sperm function.The Journal of reproduction and development · 2026Review
- Tributyltin chloride alters the structural, genomic, and epigenomic integrity of postejaculatory mammalian sperm.Epigenetics · 2025Article
- The interleukin-6 signal transducer receptor subunit is required for optimal in vitro bovine embryo development†.Biology of reproduction · 2025Article
- Emerging cooperativity between Oct4 and Sox2 governs the pluripotency network in early mouse embryos.eLife · 2025Article
- Optimization of bovine oocyte cryopreservation: membrane fusion competence and cell death of linoleic acid-Frontiers in veterinary science · 2025Article
- Transcriptomic effects of alginate hydrogel applied to the production of bovine embryos.Heliyon · 2024Article
- Molecular cloning of PRD-like homeobox genes expressed in bovine oocytes and early IVF embryos.BMC genomics · 2024Article
- Integrative pan-cancer analysis reveals a common architecture of dysregulated transcriptional networks characterized by loss of enhancer methylation.PLoS computational biology · 2024Article
- Opportunities for CRISPR-Cas9 application in farm animal genetic improvement.Molecular biology reports · 2024Review
- Embryo and fetal gene editing: Technical challenges and progress toward clinical applications.Molecular therapy. Methods & clinical development · 2024Review
- Article
- MicroRNA-148b secreted by bovine oviductal extracellular vesicles enhance embryo quality through BPM/TGF-beta pathway.Biological research · 2024Article
- Protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes.STAR protocols · 2024Article
- Optimising Electroporation Condition for CRISPR/Cas-Mediated Knockout in Zona-Intact Buffalo Zygotes.Animals : an open access journal from MDPI · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
POU5F1 is a transcription factor and master regulator of cell pluripotency with indispensable roles in early embryo development and cell lineage specification. The role of embryonic POU5F1 in blastocyst formation and cell lineage specification differs between mammalian species but remains completely unknown in cattle. The CRISPR/Cas9 system was utilized for targeted disruption of the POU5F1 gene by direct injection into zygotes. Disruption of the bovine POU5F1 locus prevented blastocyst formation and was associated with embryonic arrest at the morula stage. POU5F1 knockout morulas developed at a similar rate as control embryos and presented a similar number of blastomeres by day 5 of development. Initiation of SOX2 expression by day 5 of development was not affected by lack of POU5F1. On the other hand, CDX2 expression was aberrant in embryos lacking POU5F1. Notably, the phenotype observed in bovine POU5F1 knockout embryos reveals conserved functions associated with loss of human embryonic POU5F1 that differ from Pou5f1- null mice. The similarity observed in transcriptional regulation of early embryo development between cattle and humans combined with highly efficient gene editing techniques make the bovine a valuable model for human embryo biology with expanded applications in agriculture and assisted reproductive technologies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.