ArticleScientific reports2018
New insights into mechanisms of material ejection in MALDI mass spectrometry for a wide range of spot sizes.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- MALDI spatial proteomics: a mini review of approaches and techniques.Analytical methods : advancing methods and applications · 2026Review
- Influence of Acceleration Field and Inlet Temperature on Ion Yields of Small Molecules in Atmospheric Pressure MALDI.International journal of mass spectrometry · 2026Article
- Mass Spectrometry Imaging.Analytical chemistry · 2025Review
- Desorption and ablation regimes in UV-MALDI: the critical fluence.RSC advances · 2022Article
- Surface-assisted laser desorption/ionization mass spectrometry imaging: A review.Mass spectrometry reviews · 2022Review
- Spatially Resolved Mass Spectrometry at the Single Cell: Recent Innovations in Proteomics and Metabolomics.Journal of the American Society for Mass Spectrometry · 2021Review
- Effect of MALDI matrices on lipid analyses of biological tissues using MALDI-2 postionization mass spectrometry.Journal of mass spectrometry : JMS · 2020Article
- MALDI-2 for the Enhanced Analysis ofAnalytical chemistry · 2020Article
- The Influence of MS Imaging Parameters on UV-MALDI Desorption and Ion Yield.Journal of the American Society for Mass Spectrometry · 2019Article
- Imaging Isomers on a Biological Surface: A Review.Mass spectrometry (Tokyo, Japan) · 2019Article
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Authors and funding
2 authors.
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Abstract
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is widely used for the analysis of large biomolecules in numerous applications. The technique utilizes nanosecond-long laser pulses at various spot sizes to eject and ionize large molecules embedded in a highly absorptive chemical matrix. Despite the methods name, 'molecular desorption' from the matrix crystal surface is not the sole mechanism discussed for material ejection in MALDI, but additional ablation of larger clusters has been reported. Here we present results on the influence of laser fluence and spot size on the mechanisms of the initial material ejection in MALDI and subsequent plume development. We used a laser-based postionization (MALDI-2) as well as a complementary photoacoustic method to monitor the material ejection step. The photoacoustic data reveal a quasi-thermal sublimation process up to a transition fluence. Above this threshold fluence additional ablation processes are observed. Complementary investigations on plume dynamics by MALDI-2 showed an ejection of predominantly fast particles for desorption conditions while ablation produces considerably slower ejecta. Additionally the presented results revealed a peculiar influence of the spot size on analyte fragmentation as well as plume development and allows for new insights into the unexplained spot size effect reported for MALDI.
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