ArticleMolecular biology of the cell2018
Quantitative imaging of chromatin decompaction in living cells.
Article in Molecular biology of the cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- High-throughput DNA repair monitoring in Saccharomyces cerevisiae suggests SSB- and DSB-induced chromatin reconfiguration.Scientific reports · 2025Article
- Recent Advances in Genome Maintenance Processes.International journal of molecular sciences · 2024Article
- Real-time single-molecule imaging of transcriptional regulatory networks in living cells.Nature reviews. Genetics · 2024Review
- Transcription factor condensates and signaling driven transcription.Nucleus (Austin, Tex.) · 2023Review
- Differences in nanoscale organization of regulatory active and inactive human chromatin.Biophysical journal · 2022Article
- Regulation of RNA polymerase II activity is essential for terminal erythroid maturation.Blood · 2021Article
- Ras isoform-specific expression, chromatin accessibility, and signaling.Biophysical reviews · 2021Review
- Exercise adaptations: molecular mechanisms and potential targets for therapeutic benefit.Nature reviews. Endocrinology · 2020Review
- Imaging of DNA and RNA in Living Eukaryotic Cells to Reveal Spatiotemporal Dynamics of Gene Expression.Annual review of biochemistry · 2020Review
- Three-dimensional localization microscopy in live flowing cells.Nature nanotechnology · 2020Article
- The RNA export factor Mex67 functions as a mobile nucleoporin.The Journal of cell biology · 2019Article
- Dynamic Imaging of Transferrin Receptor Molecules on Single Live Cell with Bridge Gaps-Enhanced Raman Tags.Nanomaterials (Basel, Switzerland) · 2019Article
- Brr6 plays a role in gene recruitment and transcriptional regulation at the nuclear envelope.Molecular biology of the cell · 2018Article
- Observation of live chromatin dynamics in cells via 3D localization microscopy using Tetrapod point spread functions.Biomedical optics express · 2017Article
Corrections and comments
- Erratum issuedCorrection.2019
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Chromatin organization is highly dynamic and regulates transcription. Upon transcriptional activation, chromatin is remodeled and referred to as "open," but quantitative and dynamic data of this decompaction process are lacking. Here, we have developed a quantitative high resolution-microscopy assay in living yeast cells to visualize and quantify chromatin dynamics using the GAL7-10-1 locus as a model system. Upon transcriptional activation of these three clustered genes, we detect an increase of the mean distance across this locus by >100 nm. This decompaction is linked to active transcription but is not sensitive to the histone deacetylase inhibitor trichostatin A or to deletion of the histone acetyl transferase Gcn5. In contrast, the deletion of SNF2 (encoding the ATPase of the SWI/SNF chromatin remodeling complex) or the deactivation of the histone chaperone complex FACT lead to a strongly reduced decompaction without significant effects on transcriptional induction in FACT mutants. Our findings are consistent with nucleosome remodeling and eviction activities being major contributors to chromatin reorganization during transcription but also suggest that transcription can occur in the absence of detectable decompaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.