ArticleMicrocirculation (New York, N.Y. : 1994)2018
Establishment and characterization of an embryonic pericyte cell line.
Article in Microcirculation (New York, N.Y. : 1994), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Review
- Pericyte and Endothelial Cell Responses within Murine Cerebral Capillaries After Blood Flow Cessation.bioRxiv : the preprint server for biology · 2025Article
- In vivo transplantation of mammalian vascular organoids onto the chick chorioallantoic membrane reveals the formation of a hierarchical vascular network.Scientific reports · 2025Article
- Pericyte Progenitor Coupling to the Emerging Endothelium During Vasculogenesis via Connexin 43.Arteriosclerosis, thrombosis, and vascular biology · 2022Article
- Article
- From Donor to the Lab: A Fascinating Journey of Primary Cell Lines.Frontiers in cell and developmental biology · 2021Review
- Distinct Fibroblast Lineages Give Rise to NG2+ Pericyte Populations in Mouse Skin Development and Repair.Frontiers in cell and developmental biology · 2021Article
- Pericytes in Vascular Development.Current tissue microenvironment reports · 2020Article
- Sirtuin 3 is essential for hypertension-induced cardiac fibrosis via mediating pericyte transition.Journal of cellular and molecular medicine · 2020Article
- Differential functional roles of fibroblasts and pericytes in the formation of tissue-engineered microvascular networks in vitro.NPJ Regenerative medicine · 2020Article
- The pericyte microenvironment during vascular development.Microcirculation (New York, N.Y. : 1994) · 2019Review
- Excess vascular endothelial growth factor-A disrupts pericyte recruitment during blood vessel formation.Angiogenesis · 2019Article
- Microvascular bioengineering: a focus on pericytes.Journal of biological engineering · 2019Review
- [Research Advances of Ang-2 in Non-small Cell Lung Cancer].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2018Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
objectivePericytes are specialized perivascular cells embedded within the basement membrane. These cells envelope the abluminal surface of endothelial cells and promote microvessel homeostasis. Recent discoveries of unique pericyte functions, particularly in neural tissues, underscore the need for overcoming existing challenges in establishing a functionally validated pericyte cell line. Here, we present methodologies for addressing these challenges as well as an embryonic pericyte cell line for use with in vitro and ex vivo experimental models.
methodsWe isolated an enriched population of NG2:DsRed+ pericytes from E12.5 mice. This pericyte cell line was compared to MEFs with respect to gene expression, cell morphology and migration, and engagement with endothelial cells during junction stabilization and angiogenesis.
resultsNG2+ pericytes displayed gene expression patterns, cell morphology, and 2D migration behaviors distinct from MEFs. In three different vessel formation models, pericytes from this line migrated to and incorporated into developing vessels. When co-cultured with HUVECs, these pericytes stimulated more robust VE-Cadherin junctions between HUVECs as compared to MEFs, as well as contributed to HUVEC organization into primitive vascular structures.
conclusionsOur data support use of this pericyte cell line in a broad range of models to further understand pericyte functionality during normal and pathological conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.