Evidence map›Paper›PMID 29770347›Full record

ArticleBio-protocol2018

Precision Tagging: A Novel Seamless Protein Tagging by Combinational Use of Type II and Type IIS Restriction Endonucleases.

Zhen Xu, Yan-Ning Rui, John P Hagan, Dong H Kim

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Zhen XuDepartment of Neurosurgery, the University of Texas Health Science Center at Houston, Texas, USA.
Yan-Ning RuiDepartment of Neurosurgery, the University of Texas Health Science Center at Houston, Texas, USA.
John P HaganDepartment of Neurosurgery, the University of Texas Health Science Center at Houston, Texas, USA.
Dong H KimDepartment of Neurosurgery, the University of Texas Health Science Center at Houston, Texas, USA.
The University of Texas Health Science Center · US

Funding

The Role of THSD1 in Endothelial Cell Adhesion and SurvivalR03NS087416 · NINDS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI KIM, DONG H · 2014 to 2015
$154k
NINDS NIH HHS R03 NS087416
6 · The paper itself

Abstract

Protein tagging is a powerful tool for performing comprehensive analyses of the biological functions of a protein of interest owing to the existence of a wide variety of tags. It becomes indispensable in some cases, such as in tracking protein dynamics in a live cell or adding a peptide epitope due to the lack of optimal antibodies. However, efficiently integrating an array of tags into the gene of interest remains a challenge. Traditional DNA recombinant technology based on type II restriction endonucleases renders protein tagging tedious and inefficient as well as the introduction of an unwanted junction sequence. In our attempt to tag Thrombospondin type 1 domain-containing 1 (THSD1) that we identified as the first intracranial aneurysm gene (Santiago-Sim

Indexed as

DNA cloningNon-palindromicProtein taggingTHSD1Type IIS restriction enzyme

Identifiers

PMID29770347
PMCPMC5951406
OpenAlexW2793373334

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.