ArticleScientific reports2018
X-ray based virtual histology allows guided sectioning of heavy ion stained murine lungs for histological analysis.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 52 citations in OpenAlex.
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- Formaldehyde vapour fixation enables multiscale phase-contrast imaging and histological validation of human-sized lungs.Scientific reports · 2025Article
- Integrated 3D imaging of FFPE lung tissue combining microCT, light and electron microscopy allows for contextualized ultrastructural and histological analysis.Scientific reports · 2025Article
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- A semi-automatic pipeline integrating histological and µCT data in a mouse model of lung fibrosis.Journal of translational medicine · 2024Article
- The potential of x-ray virtual histology in the diagnosis of skin tumors.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2024Article
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- Effects of ligandrol as a selective androgen receptor modulator in a rat model for osteoporosis.Journal of bone and mineral metabolism · 2023Article
- Spatial correlation of 2D hard-tissue histology with 3D microCT scans through 3D printed phantoms.Scientific reports · 2023Article
- Selective Androgen Receptor Modulators Combined with Treadmill Exercise Have No Bone Benefit in Healthy Adult Rats.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Treatment of osteoporosis using a selective androgen receptor modulator ostarine in an orchiectomized rat model.Endocrine · 2023Article
- High resolution propagation-based lung imaging at clinically relevant X-ray dose levels.Scientific reports · 2023Article
- A combined treatment with selective androgen and estrogen receptor modulators prevents bone loss in orchiectomized rats.Journal of endocrinological investigation · 2022Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Examination of histological or immunohistochemically stained 2D sections of embedded tissue is one of the most frequently used tools in biomedical research and clinical routine. Since to date, targeted sectioning of specific regions of interest (ROI) in the sample is not possible, we aimed at developing a guided sectioning approach based on x-ray 3D virtual histology for heavy ion stained murine lung samples. For this purpose, we increased the contrast to noise ratio of a standard benchtop microCT by 5-10-fold using free-propagation phase contrast imaging and thus substantially improved image quality. We then show that microCT 3D datasets deliver more precise anatomical information and quantification of the sample than traditional histological sections, which display deformations of the tissue. To quantify these deformations caused by sectioning we developed the "Displacement Index (DI)", which combines block-matching with the calculation of the local mutual information. We show that the DI substantially decreases when a femtosecond laser microtome is used for sections as opposed to a traditional microtome. In conclusion, our microCT based virtual histology approach can be used as a supplement and a guidance tool for traditional histology, providing 3D measurement capabilities and offering the ability to perform sectioning directly at an ROI.
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