Evidence map›Paper›PMID 29767342›Full record

ArticleMolecular biology reports2018

Characterization of xanthine dehydrogenase and aldehyde oxidase of Marsupenaeus japonicus and their response to microbial pathogen.

Yo Okamura, Mari Inada, Gehad Elsaid Elshopakey, Toshiaki Itami

Abstract read
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In one paragraph

Article in Molecular biology reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Anti-salmonella properties of kefir yeast isolatesSaudi journal of biological sciences · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Yo OkamuraDepartment of Marine Biology and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan.ORCID http://orcid.org/0000-0001-9388-8202
Mari InadaNational Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, Minami-ise, Mie, 516-0193, Japan.ORCID http://orcid.org/0000-0001-6969-5558
Gehad Elsaid ElshopakeyDepartment of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.ORCID http://orcid.org/0000-0003-2924-9670
Toshiaki ItamiDepartment of Marine Biology and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan. itamit@cc.miyazaki-u.ac.jp.
University of Miyazaki · JPJapan Fisheries Research and Education Agency · JPMansoura University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reactive oxygen species (ROS) play key roles in many physiological processes. In particular, the sterilization mechanism of bacteria using ROS in macrophages is a very important function for biological defense. Xanthine dehydrogenase (XDH) and aldehyde oxidase (AOX), members of the molybdo-flavoenzyme subfamily, are known to generate ROS. Although these enzymes occur in many vertebrates, some insects, and plants, little research has been conducted on XDHs and AOXs in crustaceans. Here, we cloned the entire cDNA sequences of XDH (MjXDH: 4328 bp) and AOX (MjAOX: 4425 bp) from Marsupenaeus japonicus (kuruma shrimp) using reverse transcriptase-polymerase chain reaction (RT-PCR) and random amplification of cDNA ends (RACE). Quantitative real-time RT-PCR transcriptional analysis revealed that MjXDH mRNA is highly expressed in heart and stomach tissues, whereas MjAOX mRNA is highly expressed in the lymphoid organ and intestinal tissues. Furthermore, expression of MjAOX was determined to be up-regulated in the lymphoid organ in response to Vibrio penaeicida at 48 and 72 h after injection; in contrast, hydrogen peroxide (H

Indexed as

Aldehyde OxidaseAmino Acid SequenceAnimalsBase SequenceCloning, MolecularDNA, ComplementaryGene Expression ProfilingHydrogen PeroxideImmunity, InnatePenaeidaeReactive Oxygen SpeciesReal-Time Polymerase Chain ReactionSequence AlignmentShellfishVibrioWhite spot syndrome virus 1Aldehyde OxidaseDNA, ComplementaryHydrogen PeroxideReactive Oxygen SpeciesXanthine DehydrogenaseCloningMolybdo-flavoenzymesReactive oxygen speciesShrimp immune genesVibrio penaeicidaWhite spot syndrome virus

Identifiers

PMID29767342
OpenAlexW2803522670

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.